Serodiagnosis of Herpes Simplex Virus-1 Infections by a Prepared Antigen

For diagnosis of Herpes simplex virus (HSV) primary infections by use of complement fixation test, the HSV-CF antigen was prepared. Virus was isolated from herpetic lesions on lips and confirmed by electron microscopy and specific antisera. The results confirmed correct HSV isolation. The isolated v...

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Bibliographic Details
Published inArchives of Razi Institute Vol. 51; pp. 123 - 127
Main Authors Shahkarami, M.K., Razi Vaccine and Serum Research Institute,Tehran, Iran, Mirchamsy, H., Razi Vaccine and Serum Research Institute,Tehran, Iran, Shafieei, A., Razi Vaccine and Serum Research Institute,Tehran, Iran, Kargar moakhar, R., Razi Vaccine and Serum Research Institute,Tehran, Iran
Format Journal Article
LanguageEnglish
Published 2000
Subjects
ANTIGENE
ANTIGENOS
ANTIGENS
CELLS
CELLULE
CELULAS
COMPLEMENT FIXATION TESTS
ENCEFALITIS
ENCEPHALITE
ENCEPHALITIS
EQUIPO DE LABORATORIO
HERPESVIRIDAE
INFECCION
INFECTION
LABORATORY EQUIPMENT
MATERIEL DE LABORATOIRE
PRUEBAS DE FIJACION DEL COMPLEMENTO
REACTION DE FIXATION DU COMPLEMENT
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Summary:For diagnosis of Herpes simplex virus (HSV) primary infections by use of complement fixation test, the HSV-CF antigen was prepared. Virus was isolated from herpetic lesions on lips and confirmed by electron microscopy and specific antisera. The results confirmed correct HSV isolation. The isolated virus inoculated to susceptible cell line-Vero-with a high infections dose (MOI=0.1) and incubated at 33؛C. When complete CPE appeared, supernatant harvested as source of antigen. 93 serum samples were tested by complement fixation test (Kolmer method) using both prepared and imported antigens. The specificity and sensitivity of the test using prepared antigen in comparison of imported one were 100% and 84.7%, respectively. Because of we prepare the antigen using only one of two serotypes of the virus, in some cases that antibody directed against other serotype in patient';s serum, false-negative results occur. This led to reduction the sensitivity of test by homemade antigen.
Bibliography:2010008048
A01
ISSN:0365-3439