Pathogenicity Study and Restriction Enzyme Profile of a Recently Isolated Infectious Bursal Disease Virus in Iran

The pathogenicity of a recent isolate of Infectious bursal disease virus (IBDV), IR499, isolated from a nonvaccinated flock with 17.5 % mortality rate in susceptible SPF chickens, chickens embryos and broilers was discussed. The molecular characterization of the virus based on the restriction fragme...

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Bibliographic Details
Published inArchives of Razi Institute Vol. 58; pp. 9 - 18
Main Authors Shoshtari, A.H., Razi Vaccine & Serum Research Institute, Tehran, Iran, Pourbakhsh, S.A., Razi Vaccine & Serum Research Institute, Tehran, Iran, Dadras, H.A.,, Faculty of Veterinary Medicine, University of Shiraz, Bahmaninejad, M.A., Razi Vaccine & Serum Research Institute, Tehran, Iran, Toroghi, R., Razi Vaccine & Serum Research Institute, Tehran, Iran
Format Journal Article
LanguageEnglish
Published 2004
Subjects
AGENT PATHOGENE VIRULENT
ENZIMA DE RESTRICCION
ENZYME DE RESTRICTION
INFECTIOUS BURSAL DISEASE VIRUS
IRAN ISLAMIC REPUBLIC
IRAN REPUBLICA ISLAMICA
IRAN REPUBLIQUE ISLAMIQUE
ORGANISMOS PATOGENOS VIRULENTOS
PATHOGENICITY
PATOGENICIDAD
POUVOIR PATHOGENE
RESTRICTION ENZYMES
RFLP
VIRULENT PATHOGENS
VIRUS BURSITE INFECTIEUSE
VIRUS BURSITIS INFECCIOSA
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Summary:The pathogenicity of a recent isolate of Infectious bursal disease virus (IBDV), IR499, isolated from a nonvaccinated flock with 17.5 % mortality rate in susceptible SPF chickens, chickens embryos and broilers was discussed. The molecular characterization of the virus based on the restriction fragment length polymorphism (RFLP) pattern was also investigated. The mortality rates were 85% and 22% in SPF and broiler chickens, respectively. The bursal weight indexes were 4.7 and 1.2 for SPF birds and 1.7 and 0.7 for susceptible broilers four and nine days post inoculation, respectively. The gross lesions were generalized including moderate to sever bursal hemorrhage. Using RT-PCR, a 643bp PCR product was amplified then nested PCR was carried out to amplify a 552bp PCR product. The 552bp product was subjected to SspI, StuI, HhaI and SacI restriction enzymes digestion which was SspI and StuI double positive and HhaI and SacI double negative. The pathogenicity and RFLP pattern finding confirmed that the IR499 virus could be classified as a very virulent IBDV.
Bibliography:L73
2009008078
ISSN:0365-3439