Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit an...

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Bibliographic Details
Published inArchives of Razi Institute Vol. 59; pp. 75 - 83
Main Authors Ghadakchi, H., 1. Razi Vaccine & Serum Research Institute. Mashed, Iran, Dadras, H., Veterinary Faculty, University of Shiraz, Pourbakhsh, S.A., Razi Vaccine & Serum Research Institute. Tehran, Iran, Hosseini, S.M.H., 4. Razi Vaccine & Serum Research Institute, Shiraz, Iran
Format Journal Article
LanguageEnglish
Published 2005
Subjects
ABSORBANCE
ABSORBANCIA
ANTIGENE
ANTIGENOS
ANTIGENS
CHICKENS
ELISA
EPREUVE D'HEMAGGLUTINATION
HAEMAGGLUTINATION TESTS
INHIBICION
INHIBITION
LABORATOIRE
LABORATORIES
LABORATORIOS
MASSACHUSETTS
MORBIDITE
MORBIDITY
MORBOSIDAD
MORTALIDAD
MORTALITE
MORTALITY
POLLO
POULET
PRUEBAS DE HEMAGLUTINACION
SEROTIPOS
SEROTYPE
SEROTYPES
TEST ELISA
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Summary:An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1ىg/ml, 1:3000 and 1:100, respectively. The optical densities (OD) were compared with a commercial ELISA kit. Also, the results of ELISA were compared to hemagglutination inhibition (HI) test. The correlation coefficient of the results of both ELISAs and HI was significant (P0.05). The developed ELISA can detect antibody against IBV and is more sensitive and suitable for screening of samples in diagnostic laboratories.
Bibliography:2009008072
L73
ISSN:0365-3439