Detection of Infectious Bronchitis Virus in Allantioc Fluid by Rapid Hemagglutination Test

To detect the presence of infectious bronchitis virus (IBV) in infected allantoic fluid (AF) of SPF embryonated eggs rapid hemagglutination (HA) activity after treatment with neuraminidase enzyme was used. Twenty IBV suspected materials were inoculated in SPF embryonated eggs via chorioallantoic cav...

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Published inArchives of Razi Institute Vol. 59; pp. 47 - 54
Main Authors Momayez, R., Razi Vaccine & serum Research Institute, Gharahkhani, P., Azad University of Karaj, Toroghi, R., Razi Vaccine & serum Research Institute, Pourbakhsh, S.A., Razi Vaccine & serum Research Institute
Format Journal Article
LanguageEnglish
Published 2005
Subjects
AGGLUTININE
AGGLUTININS
AGLUTININAS
AXENIE
BLOOD CELLS
BODY FLUIDS
CELLULE SANGUINE
CELULAS SANGUINEAS
CORONAVIRIDAE
CORONAVIRUS
ENZIMAS
ENZYME
ENZYMES
EXENCION DE PATOGENOS ESPECIFICOS
LARINGOTRAQUEITIS
LARYNGOTRACHEITE
LARYNGOTRACHEITIS
LIQUIDE BIOLOGIQUE
LIQUIDOS CORPORALES
PCR
SPECIFIC PATHOGEN FREE STATE
VIROSE
VIROSES
VIROSIS
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Summary:To detect the presence of infectious bronchitis virus (IBV) in infected allantoic fluid (AF) of SPF embryonated eggs rapid hemagglutination (HA) activity after treatment with neuraminidase enzyme was used. Twenty IBV suspected materials were inoculated in SPF embryonated eggs via chorioallantoic cavity. Harvested AFs were treated with neuraminidase enzyme and the presence of IBV was detected by inducing hemagglutination of chicken red blood cells. The specificity of rapid HA test was examined with three non-hemagglutinating avian viruses including infectious bursal disease virus, infectious laryngotracheitis virus and fowl pox virus. The sensitivity of the test was compared with reversed transcriptase-polymerase chain reaction. The results showed that this test was specific and had a sensitivity of 100% for IBV detection. The results of this study indicate that HA test using neuraminidase treatment is an accurate, sensitive, specific and inexpensive test for rapid detection of IBV.
Bibliography:L73
2009008069
ISSN:0365-3439