Genetic integrity of cryopreserved potato (Solanum tuberosum) plants

The technology of Cryopreservation is of high importance for long-term preserving of germplasm. This technology is useful only when no genetic variation is induced. In order to study genetic stability of two potato varieties, Agria and Marphona, preserved in long-term and normal conditions, AFLP tec...

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Main Authors Zarghami, Reza, Iran Nezhad, Azadeh, Dabir Ashrafi, Omid
Format Publication
LanguagePersian
Published Karaj (Iran) Agricultural Biotechnology Research Institute of Iran 2007
Subjects
Agria
BIOLOGICAL PRESERVATION
CONGELACION
CONGELATION
CONSERVACION BIOLOGICA
CONSERVATION BIOLOGIQUE
ESTABILIDAD GENETICA
FREEZING
GENETIC RESOURCES
GENETIC STABILITY
GERMOPLASMA
GERMPLASM
IRAN ISLAMIC REPUBLIC
IRAN REPUBLICA ISLAMICA
IRAN REPUBLIQUE ISLAMIQUE
Marphona
PAPA
PAPA DE SIEMBRA
PLANT DE POMME DE TERRE
POMME DE TERRE
POTATOES
PRESERVACION
PRESERVATION
RECURSOS GENETICOS
RESSOURCE GENETIQUE
SEED POTATOES
SOLANUM TUBEROSUM
STABILITE GENETIQUE
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Summary:The technology of Cryopreservation is of high importance for long-term preserving of germplasm. This technology is useful only when no genetic variation is induced. In order to study genetic stability of two potato varieties, Agria and Marphona, preserved in long-term and normal conditions, AFLP technique was used. Induced variations in ploidy level were also assessed by Flowcytometry and cytogenic studies. The result of AFLP analysis performed by 7 primer combinations, indicated 97% homology between seedlings of Agria preserved in longterm and normal conditions. Seedlings of Marfona also in two conditions showed 100% homology. Cryopreservation also induced no variation in ploidy level.
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