Comparative Assessment Of Electrophoretic Patterns Of Fasciola Somatic Antigens In Different Hosts

To determine the electrophoretic patterns of Fasciola hepatita and F. gigantica, the former was collected from sheep and buffalo infected liver and the latter from that of cattle and camel and were washed in PBS. Somatic antigens of both species were prepared though homogenization and centrifugation...

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Published inPajouhesh And Sazandegi Vol. 20; no. 2
Main Authors Meshgi, B., Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Eslami, A., Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Hossieni, S.H., Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Hemmatzadeh, F., Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Hooshmand, E., Faculty of Agriculture, Eslamic Azad University, Rasht, Iran
Format Journal Article
LanguagePersian
Published 2008
Subjects
ANALYTICAL METHODS
ANTIGENE
ANTIGENOS
ANTIGENS
BETAIL
CAPRINAE
CELLS
CELLULE
CELULAS
ELECTROFORESIS
ELECTROPHORESE
ELECTROPHORESIS
FASCIOLA
FASCIOLA GIGANTICA
FASCIOLA HEPATICA
FASCIOLIDAE
GANADO
HOSTS
HOTE
HUESPEDES
IRAN ISLAMIC REPUBLIC
IRAN REPUBLICA ISLAMICA
IRAN REPUBLIQUE ISLAMIQUE
LIVESTOCK
OVIN
OVINOS
PLATYHELMINTHES
SHEEP
Somoticantigen
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
TREMATODA
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Summary:To determine the electrophoretic patterns of Fasciola hepatita and F. gigantica, the former was collected from sheep and buffalo infected liver and the latter from that of cattle and camel and were washed in PBS. Somatic antigens of both species were prepared though homogenization and centrifugation of adult flukes. To show the eclrophoretic patterns of somatic antigens, the prepared antigens were electrophoresed using SDS-PAGE. Our findings revealed that proteins of 18-70 kDa molecular weights were present in both species in different hosts. Although a 57 kDa protein was seen in Fasciola gigantica of cattle and camel only. Meanwhile two bands of 54 and 68 kDa were seen exclusively in F.hepatica of sheep and buffalo. A band of 64 kDa was shown in F.hepatica of buffalo. According to our results in any study on immunological or immunodiagnostic of Fasciola spp, the species trematode as well as their hosts should be taken in consideration.
Bibliography:L70
2008003021
L01
ISSN:1019-9632