An efficient method of micropropagation of ginger (Zingiber officinale Rosc. Cv. Suprava and Suruchi) through in vitro rhizome bud culture
An efficient, simple micropropagation method was developed for Zingiber officinale Rose. (cv, Suprava and Suruchi) using fresh rhizome sprouting bud in semisolid culture media. Explants were cultured on Murashige and Skoog's (MS) medium supplemented with different concentrations and combinat~on...
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Published in | Indian journal of plant physiology Vol. 14; no. 2 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
01.04.2009
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Subjects | |
Online Access | Get more information |
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Summary: | An efficient, simple micropropagation method was developed for Zingiber officinale Rose. (cv, Suprava and Suruchi) using fresh rhizome sprouting bud in semisolid culture media. Explants were cultured on Murashige and Skoog's (MS) medium supplemented with different concentrations and combinat~ons of RAP (6-benzyl- amino-purine) and NAA (a- naphthalene acetic acid) for shoot and root induction. Explants cultured on MS basal medium supplemented with 2.0 mgll RAP + 0.5mgll NAA showed highest rate of shoot multiplication. In vitro shoots were rooted on to the half strength MS basal medium supplemented with 2.0 mgll NAA and rooting was better. Rooted shoots were transplanted in the green house for hardening and their survival was 95 % in the field condition. |
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Bibliography: | F60 2010001127 F02 |
ISSN: | 0019-5502 0974-0252 |