Production of embryoids from anthers of Pometia pinnata: a tropical forest tree

• Main Authors: Sudarmonowati, E, Yunita, E. (Pusat Penelitian dan Pengembangan Bioteknologi, Jakarta (Indonesia))
• Format: Conference Proceeding
• Language: English
• Published: Bogor (Indonesia) IPB 1997
• Subjects: ANA; ANTHER CULTURE; BOSQUE TROPICAL; CAL; CALLO; CALLUS; CULTIVO DE ANTERAS; CULTURE D'ANTHERE; DESARROLLO EMBRIONARIO; DEVELOPPEMENT EMBRYONNAIRE; EMBRYONIC DEVELOPMENT; FACTEUR DE CROISSANCE; FACTORES DE CRECIMIENTO; FORET TROPICALE; GROWTH FACTORS; NAA; POMETIA; TROPICAL FORESTS
• ISBN: 9799552605; 9789799552600

Anther culture of Pometia pinnata as a model of tropical forest tree species has been developed as an effort to shorten a lengthy breeding procedure through conventional techniques. The success rate of anther culture in many species especially forest tree species was very low. Factors affecting the production of embryoids from anther culture such the composition of culture medium including type of gelling agent, anther stage, pretreatment conditions and period of incubation were investigated. The highest percentage of explants producing callus (26.7 percent) which all of them could produce embryoids was obtained from the youngest stage of anthers which was yellow in colour cultured on modified WP induction callus medium supplemented with 10 mg/l 2,4-D, 6 percent sucrose, 1.75 percent glucose, 100 mg/l ficoll and solidified with 0.6 percent agarose following precultures on MS liquid media containing the same composition as induction medium expect for 2,4-D which was 8 mg/l. Pretreating the anthers at low temperatures has a negative effect on callus production. Embryoids obtained could be converted to plantlets when they were subjected to a series of transfer on WP medium containing a combination of 1.0 mg/l kinetin and 0.01 mg/l NAA. Effort to increase the conversion of embryoids to normal plantlets is still in progress