Measuring the concentration of Infectious Bursal Disease virus (IBDV) antigen by means of sandwich ELISA

Author developed a sandwich ELISA procedure for measuring the concentration of IBDV antigen so as to follow viral multiplication in the sourse of vaccine production. Purification of detecting antibody by affinity chromatography eliminated background reactions in a large measure. Immunoassay sensitiv...

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Bibliographic Details
Published inPhylaxia Allatorvosi Kozlemenyek Vol. 25
Main Authors Kardi V, Perenyi T, Kocsis Gy, Neumayer Gy, Varro Cs, Nagy B.F, Pergel I
Format Journal Article
LanguageHungarian
Published 1989
Subjects
antigene
antigenos
antigens
avian infectious bursitis
chickens
elisa
enfermedad de gumboro
maladie de gumboro
pollo
poulet
test elisa
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Summary:Author developed a sandwich ELISA procedure for measuring the concentration of IBDV antigen so as to follow viral multiplication in the sourse of vaccine production. Purification of detecting antibody by affinity chromatography eliminated background reactions in a large measure. Immunoassay sensitivity was found to be about 10000 TCID50/0.1 ml. The immunoassay provides a simple, rapid method for following virus purification and concentration. Results of infectivity titrations in cell cultures paralelled with those obtained by sandwich ELISA.
Bibliography:L73
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ISSN:0238-0315