Data in support of peptidomic analysis of spermatozoa during epididymal maturation

The final differentiation of the male germ cell occurs in the epididymal duct where the spermatozoa develop the ability to be motile and fertilize an ovum. Understanding of these biological processes is the key to understanding and controlling male fertility. Comparative studies between several epid...

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Bibliographic Details
Published inData in Brief (1), 79-84. (2014)
Main Authors Labas, Valérie, Spina, Lucie, Belleannee, Clémence, Teixeira Gomes, Ana Paula, Gargaros, Audrey, Dacheux, Françoise, Dacheux, Jean-Louis
Format Publication
LanguageEnglish
Published 2014
Subjects
analyse quantitative
Autre (Sciences du Vivant)
donnée
epididymis
maldi tof
maturation
maturation épididymaire
peptidome
peptidomique
protéome
recherche
spectrométrie de masse
spermatozoa
sperme
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Summary:The final differentiation of the male germ cell occurs in the epididymal duct where the spermatozoa develop the ability to be motile and fertilize an ovum. Understanding of these biological processes is the key to understanding and controlling male fertility. Comparative studies between several epididymal maturation states could be an informative approach to finding sperm modifications related to maturation and fertility. Here we show the data from differential peptidomic/proteomic analyses on spermatozoa isolated from 4 epididymal regions (immature to mature stage) using a profiling approach based on MALDI-TOF mass spectrometry and, combined to top-down MS in order to identify peptidoforms and proteoforms. By this way, 172m/z peaks ranging between 2 and 20 kDa were found to be modified during maturation of sperm. A total of 62m/z were identified corresponding to 32 different molecular species.
Bibliography:http://prodinra.inra.fr/record/277096
10.1016/j.dib.2014.10.003
http://prodinra.inra.fr/ft/2D4B496E-1FE2-4969-9FCC-7FA8F6A227E0