Additive inheritance of histone modifications in Arabidopsis thaliana intra-specific hybrids

• Published in: http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291365-313X Vol. 4
• Main Authors: Banaei Moghaddam , Ali M. (Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben(Allemagne).), Roudier , François (Centre National de la Recherche ScientifiqueInstitut de Biologie de l’Ecole Normale SupérieureInstitut National de la Santé et de la Recherche Médicale, PARISParisparis(France). UMR8197U1024), Seifert , Michael (Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben(Allemagne).), Berard , Caroline (INRA , Paris (France). UMR 0518 Mathématiques et Informatique Appliquées), Magniette , Marie-Laure (INRA , Paris (France). UMR 0518 Mathématiques et Informatique Appliquées), Karimi Ashtiyani , Raheleh (Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben(Allemagne).), Houben , Andreas (Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben(Allemagne).), Colot , Vincent (Institut de Biologie de l’Ecole Normale SupérieureCentre National de la Recherche ScientifiqueInstitut National de la Santé et de la Recherche Médicale, ParisPARISparis(France). UMR8197U1024)
• Format: Publication
• Language: English
• Published: 2011
• Subjects: arabidopsis thaliana; chip on chip; coton; cotton; cytosine; cytosine methylation; dna methylation; elements; epigenome; genes; genome; génome; heterosis; histone methylation; hybride intraspécifique; hétérosis; intra-specific hybrids; modification patterns; méthylation de l'adn; plants; retrotransposons; rétrotransposon; tiling microarrays; élément; épigénome

Plant genomes are earmarked with defined patterns of chromatin marks. Little is known about the stability of these epigenomes when related, but distinct genomes are brought together by intra-species hybridization. Arabidopsis thaliana accessions and their reciprocal hybrids were used as a model system to investigate the dynamics of histone modification patterns. The genome-wide distribution of histone modifications H3K4me2 and H3K27me3 in the inbred parental accessionsCol-0, C24 and Cvi and their hybrid offspring was compared by chromatin immunoprecipitation in combination with genome tiling array hybridization. The analysis revealed that, in addition to DNA sequence polymorphisms, chromatin modification variations exist among accessions of A. thaliana . The range of these variations was higher for H3K27me3 (typically a repressive mark) than for H3K4me2 (typically an active mark). H3K4me2 and H3K27me3 were rather stable in response to intra-species hybridization, with mainly additive inheritance in hybrid offspring. In conclusion, intra-species hybridization does not result in gross changes to chromatin modifications.