Transcriptome profiling of Chironomus kiinensis under phenol stress using Solexa sequencing technology

• Published in: http://www.plosone.org Vol. 3
• Main Authors: Cao , Chuanwang (Harbin Forestry University, Harbin(Chine). Forest Protection Department), Wang , Zhiying (Harbin Forestry university, Harbin(Chine). Forest Protection Department), Niu , Changying (Huazhong Agricultural University, Wuhan(Chine). Insect Resources Utilization and Sustainable Pest Management Key Laboratory), Desneux , Nicolas (INRA , Biot (France). UMR 1355 Institut Sophia Agrobiotech ), Gao , Xiwu(auteur de correspondance) (China Agricultural University, Beijing(Chine). Department of Entomology)
• Format: Publication
• Language: English
• Published: 2013
• Subjects: actif; analyse comparative; analyse différentielle; analyse du transcriptome; approche transcriptomique; Chironomus kiinensis; classification fonctionnelle; enrichissement; expression des gènes; gène candidat; gène régulateur; génome; invertébré; ligand; mobilité dans l'environnement; neurorécepteur; ontologie; pancréas; phénol; polluant; profil d'expression; réaction phénolique; récepteur aryl hydrocarbone; solubilité; stabilité chimique; stress; séquençage; technique de séquençage; temps réel; voie métabolique; écosystème aquatique

Phenol is a major pollutant in aquatic ecosystems due to its chemical stability, water solubility and environmental mobility. To date, little is known about the molecular modifications of invertebrates under phenol stress.[br/] In the present study, we used Solexa sequencing technology to investigate the transcriptome and differentially expressed genes (DEGs) of midges (Chironomus kiinensis) in response to phenol stress. A total of 51,518,972 and 51,150,832 clean reads in the phenol-treated and control libraries, respectively, were obtained and assembled into 51,014 non-redundant (Nr) consensus sequences. A total of 6,032 unigenes were classified by Gene Ontology (GO), and 18,366 unigenes were categorized into 238 Kyoto Encyclopedia of Genes and Genomes (KEGG) categories.[br/] These genes included representatives from almost all functional categories. A total of 10,724 differentially expressed genes (P value <0.05) were detected in a comparative analysis of the expression profiles between phenol-treated and control C. kiinensis including 8,390 upregulated and 2,334 downregulated genes. The expression levels of 20 differentially expressed genes were confirmed by real-time RT-PCR, and the trends in gene expression that were observed matched the Solexa expression profiles, although the magnitude of the variations was different.[br/] Through pathway enrichment analysis, significantly enriched pathways were identified for the DEGs, including metabolic pathways, aryl hydrocarbon receptor (AhR), pancreatic secretion and neuroactive ligand-receptor interaction pathways, which may be associated with the phenol responses of C. kiinensis.[br/] Using Solexa sequencing technology, we identified several groups of key candidate genes as well as important biological pathways involved in the molecular modifications of chironomids under phenol stress.