ALLELIC AND GENOTYPIC FREQUENCIES OF CALPAIN, CALPASTATIN AND LEPTIN IN COLOMBIAN CREOLE CATTLE
The Calpain (CAPN1), Calpastatin (CAST) and Leptin (LEP) genes are considered of substantial importance for the production, composition and/or sanitary quality of the meat. In order to assess the allele and genotype frequencies of genes, seven SNPs were evaluated in 300 DNA samples from eight Colomb...
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Published in | Actas Iberoamericanas de Conservación Animal - AICA Vol. 2 |
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Main Authors | , , , , |
Format | Journal Article |
Language | Spanish |
Published |
Córdoba - España
Red CONBIAND
2012
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Subjects | |
Online Access | Get full text |
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Summary: | The Calpain (CAPN1), Calpastatin (CAST) and Leptin (LEP) genes are considered of substantial importance for the production, composition and/or sanitary quality of the meat. In order to assess the allele and genotype frequencies of genes, seven SNPs were evaluated in 300 DNA samples from eight Colombian Creole Cattle breeds, two synthetic breeds and 55 individuals from four commercial breeds. The allele frequencies of genes reported as associated with meat tenderness like Calpain gene were: 21% for the C allele of CAPN1316, 64% for the allele G of CAPN1530, 55% for the allele C of CAPN14751 and 41% for the allele A of CAPN15331. For Calpastatin gene the allele frequencies were: 66% for the allele A of the marker 2959 and 39% for the allele C of the marker CAST/Xmn1. On the other hand, the proportion of varying T of the gene LEP and reported to be associated with marbling was of 53%. The results indicate that the Colombian Creole Cattle besides being fully adapted to the environmental conditions, resistant to parasites and with docile temperament, they also present alleles of interest associated to meat quality traits that can be useful for programs of animal breeding and conservation.
Los genes de la Calpaína (CAPN1), la Calpastatina (CAST) y la Leptina (LEP) son de una gran importancia para la producción, composición y/o calidad sanitaria de la carne. Para tipificar las frecuencias, alélicas y genotípicas, de estos genes se evaluaron siete marcadores de tipo SNP en 300 muestras de ADN de ocho razas bovinas criollas, dos razas sintéticas colombianas y 55 muestras de cuatro razas comerciales. Las frecuencias de las variantes alélicas de interés, relacionadas con la terneza de la carne en el gen de la Calpaína fueron: 21% para el alelo C de CAPN1316, 64% para el alelo G de CAPN1530, 55% para el alelo C de CAPN14751 y 41% para el alelo A de CAPN15331. Para el gen de la Calpastatina fueron: 66% para el alelo A de 2959 y 39% para el alelo C de CAST/Xmn1. La proporción de la variante T del gen LEP, relacionada con el veteado marmóreo de la carne, fue del 53%. Los resultados indican que los bovinos criollos colombianos además de estar plenamente adaptados a las condiciones ambientales, ser resistentes a parásitos y poseer un temperamento dócil, también presentan alelos de interés en cuanto a características de calidad de la carne, que pueden ser de gran utilidad para los programas de mejoramiento y conservación. |
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Bibliography: | 06861 http://www.uco.es/conbiand/aica/templatemo_110_lin_photo/articulos/2012/Trabajo072_AICA2012.pdf |
ISSN: | 2253-7325 |