Protocol proposal for samples collection for microbiological analysis of dog faeces

• Published in: Congresos y Jornadas. Serie Producción Animal - Asociación Interprofesional para el Desarrollo Agrario (España) pp. 787 - 789
• Main Authors: González Ortiz, G., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària, Adelantado, C., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària, Gómez de Segura, A., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària, Hervera, M., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària, Arosemena, E.L., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària, Calvo, M.A., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària, Baucells, M.D., Universitat Autònoma de Barcelona, Bellaterra (España). Facultat de Veterinària
• Format: Conference Proceeding
• Language: Spanish
• Published: Zaragoza (España) AIDA 2009
• Subjects: ANALISIS MICROBIOLOGICO; ANALYSE MICROBIOLOGIQUE; CHIEN; DOGS; ECHANTILLONNAGE; FAECES; FECES; FLORA INTESTINAL; FLORE INTESTINALE; HECES; http://www.fao.org/aos/agrovoc#c_16363; http://www.fao.org/aos/agrovoc#c_2352; http://www.fao.org/aos/agrovoc#c_25302; http://www.fao.org/aos/agrovoc#c_2772; http://www.fao.org/aos/agrovoc#c_4788; http://www.fao.org/aos/agrovoc#c_6774; INTESTINAL FLORA; METHODE; METHODS; METODOS; MICROBIOLOGICAL ANALYSIS; MUESTREO; PERRO; SAMPLING
• ISBN: 8461323114; 9788461323111

Some trials which include canine digestive microbiology studies do not have a standardized methodology about faeces collection. The aim of these studies was to make a standardized collection protocol to study the common microbiota in dog faeces. Study goals were to study the repeatability in the same sample, to determine the repeatability between days and to analyze the cold storage effect on the usual microbiota. The repeatability within the faeces was confirmed on the microbiota. Only variations between days on Lactic Acid Bacteria counts were detected. No differences were found in the microbiota (Enterobacteriaceae spp, Escherichia coli and Lactic Acid Bacteria) between fresh samples and refrigerated faeces. However, we observed an increase in the counts of Clostridium perfringens when the samples were kept refrigerated in different days in the two different studies. Thus, this effect could restrict the preservation of the faecal sample in the fridge until its analysis. More than one sample by sampling period may be considered in order to minimise the microbiota variation.