Self-incompatibility gene interactions in resynthesized Brassica napus L

• Published in: Fortschritte der Pflanzenzuechtung Vol. 18
• Main Authors: Odenbach W, Beschorner M
• Format: Journal Article
• Language: English
• Published: 1995
• Subjects: autoesterilidad; autosterilite; brassica napus; gene; gene interaction; genes; interaccion de genes; interaction genique; self sterility
• ISSN: 0301-2727

Basic research has been done widely, both studying the molecular structure of at least important parts of the S-locus, and of the dominance and inter-S-loci relationships. But still a lot of questions remain open e.g. the regulation of weakness and strongness of the S-alleles, the relationship of the quantity of produced SLGs to the S-allele activity. Are there specific differences in the amount of SLG production related to Brassica campestris, and Brassoca oleracea, respectively? This question arises because in our comparative investigations of SLG patterns, B. campestris specific SLG fractions could be demonstrated in both the parental line and the respective resynthesized B. napus after Con A-linking, while this was not true for the B. oleracea specific fractions. Since it could be shown to distinguish specific B. oleracea S-alleles using six restriction enzymes and analyzing the patterns of digested PCR products it should be reasonable to use this method to discriminate between B. campestris S-alleles. Preliminary studies revealed the same B. oleracea primer to be suitable to amplify B. campestris SLG sequences. The next step in our investigations related to SI as a appropriate mechanism for hybrid oilseed rape breeding is to develop a set of restriction patterns of B. campestris SLG sequences comparable to that established for B. oleracea. This would be a tool for the breeders to identify their material rapidly.