C banding and fluorescent banding pattern of the chromosomes of cunninghamia lanceolate
对杉木的根尖有丝分裂中期染色体进行研究,结果发现,杉木的染色体核型为2n=22=20m(2SAT)+2sm,10对染色体均为中间着丝粒染色体,只有1对(最小一对)为近中着丝粒染色体,第3对为具随体的染色体,核型不对称性属于1B型。对杉木的Giemsa C-带进行研究发现,有8对染色体有C带出现,只有3对染色体无C带,C带纹均出现在染色体的两臂。且利用C带在杉木11对染色体上的分布情况,能够较容易地辨认出11对中的5对染色体。而荧光分带研究的结果则为在杉木根尖细胞的中期分裂相中,只有CMA(色霉素A3)在带有随体的染色体的次缢痕和随体处有专一的荧光带纹,而DAPI无带。CMA带比DAPI带更适宜...
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Published in | Molecular plant breeding Vol. 5; no. 4 |
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Format | Journal Article |
Language | Chinese |
Published |
01.07.2007
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Abstract | 对杉木的根尖有丝分裂中期染色体进行研究,结果发现,杉木的染色体核型为2n=22=20m(2SAT)+2sm,10对染色体均为中间着丝粒染色体,只有1对(最小一对)为近中着丝粒染色体,第3对为具随体的染色体,核型不对称性属于1B型。对杉木的Giemsa C-带进行研究发现,有8对染色体有C带出现,只有3对染色体无C带,C带纹均出现在染色体的两臂。且利用C带在杉木11对染色体上的分布情况,能够较容易地辨认出11对中的5对染色体。而荧光分带研究的结果则为在杉木根尖细胞的中期分裂相中,只有CMA(色霉素A3)在带有随体的染色体的次缢痕和随体处有专一的荧光带纹,而DAPI无带。CMA带比DAPI带更适宜杉木的分带研究。最后讨论了C带与荧光带的存杉木染色体研究中的应用。
The chromosome karyotype, C-banding, and fluorescent banding of Cunninghamia lanceolate (Chinese fir) were analyzed in this paper, The results were as follows: The karyotype of metaphase chromosome is 2n=22=20m (2SAT)+2sm, 10 pairs of chromosomes are medium centromeric chromosomes and 1 pair of chromosome is submedium centromeric chromosome. And there is 1 pair of satellites occurred on chromosome 3. Karyogramwas belong to the type of 1B. The C -banding occurred on 8 pairs of chromosomes, but it didn't occurred on 3 pairs of chromosomes. All of C banding is intercary-banding. And 5 of the 11 chromosomes of Chinese fir were easily identified by C banding. CMA banding only occurred o |
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AbstractList | 对杉木的根尖有丝分裂中期染色体进行研究,结果发现,杉木的染色体核型为2n=22=20m(2SAT)+2sm,10对染色体均为中间着丝粒染色体,只有1对(最小一对)为近中着丝粒染色体,第3对为具随体的染色体,核型不对称性属于1B型。对杉木的Giemsa C-带进行研究发现,有8对染色体有C带出现,只有3对染色体无C带,C带纹均出现在染色体的两臂。且利用C带在杉木11对染色体上的分布情况,能够较容易地辨认出11对中的5对染色体。而荧光分带研究的结果则为在杉木根尖细胞的中期分裂相中,只有CMA(色霉素A3)在带有随体的染色体的次缢痕和随体处有专一的荧光带纹,而DAPI无带。CMA带比DAPI带更适宜杉木的分带研究。最后讨论了C带与荧光带的存杉木染色体研究中的应用。
The chromosome karyotype, C-banding, and fluorescent banding of Cunninghamia lanceolate (Chinese fir) were analyzed in this paper, The results were as follows: The karyotype of metaphase chromosome is 2n=22=20m (2SAT)+2sm, 10 pairs of chromosomes are medium centromeric chromosomes and 1 pair of chromosome is submedium centromeric chromosome. And there is 1 pair of satellites occurred on chromosome 3. Karyogramwas belong to the type of 1B. The C -banding occurred on 8 pairs of chromosomes, but it didn't occurred on 3 pairs of chromosomes. All of C banding is intercary-banding. And 5 of the 11 chromosomes of Chinese fir were easily identified by C banding. CMA banding only occurred o |
Author | Xu Jin, Shi Jisen |
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Snippet | 对杉木的根尖有丝分裂中期染色体进行研究,结果发现,杉木的染色体核型为2n=22=20m(2SAT)+2sm,10对染色体均为中间着丝粒染色体,只有1对(最小一对)为近中着丝粒染色体,第3对为具随体的染色体,核型不对称性属于1B型。对杉木的Giemsa... |
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Title | C banding and fluorescent banding pattern of the chromosomes of cunninghamia lanceolate |
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