C banding and fluorescent banding pattern of the chromosomes of cunninghamia lanceolate

• Published in: Molecular plant breeding Vol. 5; no. 4
• Main Author: Xu Jin, Shi Jisen
• Format: Journal Article
• Language: Chinese
• Published: 01.07.2007
• Subjects: CELLS; CELLULE; CELULAS; CHROMOSOME; CHROMOSOMES; CROMOSOMAS; CUNNINGHAMIA; http://www.fao.org/aos/agrovoc#c_1418; http://www.fao.org/aos/agrovoc#c_1598; http://www.fao.org/aos/agrovoc#c_32107
• ISSN: 1672-416X

对杉木的根尖有丝分裂中期染色体进行研究，结果发现，杉木的染色体核型为2n=22=20m（2SAT）＋2sm，10对染色体均为中间着丝粒染色体，只有1对（最小一对）为近中着丝粒染色体，第3对为具随体的染色体，核型不对称性属于1B型。对杉木的Giemsa C-带进行研究发现，有8对染色体有C带出现，只有3对染色体无C带，C带纹均出现在染色体的两臂。且利用C带在杉木11对染色体上的分布情况，能够较容易地辨认出11对中的5对染色体。而荧光分带研究的结果则为在杉木根尖细胞的中期分裂相中，只有CMA（色霉素A3）在带有随体的染色体的次缢痕和随体处有专一的荧光带纹，而DAPI无带。CMA带比DAPI带更适宜杉木的分带研究。最后讨论了C带与荧光带的存杉木染色体研究中的应用。 The chromosome karyotype, C-banding, and fluorescent banding of Cunninghamia lanceolate （Chinese fir） were analyzed in this paper, The results were as follows： The karyotype of metaphase chromosome is 2n=22=20m （2SAT）＋2sm, 10 pairs of chromosomes are medium centromeric chromosomes and 1 pair of chromosome is submedium centromeric chromosome. And there is 1 pair of satellites occurred on chromosome 3. Karyogramwas belong to the type of 1B. The C -banding occurred on 8 pairs of chromosomes, but it didn＇t occurred on 3 pairs of chromosomes. All of C banding is intercary-banding. And 5 of the 11 chromosomes of Chinese fir were easily identified by C banding. CMA banding only occurred o