Construction and verification of double T-DNA plant expression vector of the LFY gene
LFY基因是从从野生型拟南芥中克隆出的一个与调控花分生组织启动相关的基因,它有促进植物提早开花的作用。用PCR方法从克隆载体上扩增出LFY基因并在两端添加XhoⅠ酶切位点,将其连接在用XhoⅠ切除了hyg基因的pCAMBIA1301表达载体上,得到去除选择标记的LFY基因植物表达载pCAM-BIA1301-LFY。质粒pCAMBIA1301经HindⅡ/EcoRⅠ双酶切补平自连后得到去除多克隆位点的质粒pCAMBIA1301-,再经SacII/SphI双酶切后插入到经SacⅡ酶切的pCAMBIA1301-LFY中。建成双T-DNA植物表达载体pCAMBIA1301-LFY-hyg-,经酶切鉴定...
Saved in:
| Published in | Molecular plant breeding Vol. 14; no. 2 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
01.03.2006
|
| Subjects | |
| Online Access | Get more information |
Cover
Loading…
| Abstract | LFY基因是从从野生型拟南芥中克隆出的一个与调控花分生组织启动相关的基因,它有促进植物提早开花的作用。用PCR方法从克隆载体上扩增出LFY基因并在两端添加XhoⅠ酶切位点,将其连接在用XhoⅠ切除了hyg基因的pCAMBIA1301表达载体上,得到去除选择标记的LFY基因植物表达载pCAM-BIA1301-LFY。质粒pCAMBIA1301经HindⅡ/EcoRⅠ双酶切补平自连后得到去除多克隆位点的质粒pCAMBIA1301-,再经SacII/SphI双酶切后插入到经SacⅡ酶切的pCAMBIA1301-LFY中。建成双T-DNA植物表达载体pCAMBIA1301-LFY-hyg-,经酶切鉴定证明了所构建载体的正确性。将此双T-DNA载体用农杆菌介导法转化菊花叶片,期望得到无选择标记含LFY基因的转基因菊花。 |
|---|---|
| AbstractList | LFY基因是从从野生型拟南芥中克隆出的一个与调控花分生组织启动相关的基因,它有促进植物提早开花的作用。用PCR方法从克隆载体上扩增出LFY基因并在两端添加XhoⅠ酶切位点,将其连接在用XhoⅠ切除了hyg基因的pCAMBIA1301表达载体上,得到去除选择标记的LFY基因植物表达载pCAM-BIA1301-LFY。质粒pCAMBIA1301经HindⅡ/EcoRⅠ双酶切补平自连后得到去除多克隆位点的质粒pCAMBIA1301-,再经SacII/SphI双酶切后插入到经SacⅡ酶切的pCAMBIA1301-LFY中。建成双T-DNA植物表达载体pCAMBIA1301-LFY-hyg-,经酶切鉴定证明了所构建载体的正确性。将此双T-DNA载体用农杆菌介导法转化菊花叶片,期望得到无选择标记含LFY基因的转基因菊花。 |
| Author | Sun Lei, Zhang Qixiang |
| Author_xml | – sequence: 1 fullname: Sun Lei, Zhang Qixiang |
| BookMark | eNqFjMEKgkAURWdhkJWfELwfEGZMdB2WtIhWBbWSUd_YgLyRmVH6_CzaBxcu3HO4KxaQIQxYKLI8iVOR3Zcsck7XnAsxR6QhuxWGnLdj47UhkNTChFYr3cjvYBS0Zqx7hGt8uOxh6CV5wNdgcT6ahQkbb-zH80-Ec_mADgk3bKFk7zD69Zpty-O1OMVKmkp2VruqoITznPOE79LkH38D4Bc8FQ |
| ContentType | Journal Article |
| DBID | FBQ |
| DatabaseName | AGRIS |
| DatabaseTitleList | |
| Database_xml | – sequence: 1 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
| DeliveryMethod | no_fulltext_linktorsrc |
| DocumentTitleAlternate | LFY基因双T-DNA植物表达载体的构建 |
| ExternalDocumentID | Cn2007002034 |
| GroupedDBID | 2B. 2B~ 92G 92I ACGFS ALMA_UNASSIGNED_HOLDINGS CW9 FBQ TCJ TGD U1G U5N |
| ID | FETCH-fao_agris_Cn20070020342 |
| ISSN | 1672-416X |
| IngestDate | Tue Nov 07 23:25:42 EST 2023 |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 2 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-fao_agris_Cn20070020342 |
| Notes | F30 2007002034 |
| ParticipantIDs | fao_agris_Cn2007002034 |
| PublicationCentury | 2000 |
| PublicationDate | Mar. 2006 |
| PublicationDateYYYYMMDD | 2006-03-01 |
| PublicationDate_xml | – month: 03 year: 2006 text: Mar. 2006 |
| PublicationDecade | 2000 |
| PublicationTitle | Molecular plant breeding |
| PublicationYear | 2006 |
| SSID | ssib001101114 ssib002263098 ssib006567417 ssib051370153 ssj0000651139 |
| Score | 3.3939784 |
| Snippet | ... |
| SourceID | fao |
| SourceType | Publisher |
| SubjectTerms | CHRYSANTHEME CHRYSANTHEMUMS CRISANTEMOS GENE TRANSFER GENETIC VECTORS http://www.fao.org/aos/agrovoc#c_24846 http://www.fao.org/aos/agrovoc#c_27532 http://www.fao.org/aos/agrovoc#c_33342 TRANSFERENCIA DE GENES TRANSFERT DE GENE VECTEUR GENETIQUE VECTORES GENETICOS |
| Title | Construction and verification of double T-DNA plant expression vector of the LFY gene |
| Volume | 14 |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ3PS8MwFMeDPy5eRNHhr0kO3kbFtGm7Hud0DNkGwgbzNNqsmV66ywTZX-_3pWsTZYJ6KSVNS5tPePnm5aWPsRup5-0wj3JvTouEUmTSS2OhvUyFicoTqVRGfsjhKOpP5NM0nNrsrmZ3ySq7Veut-0r-QxVl4Eq7ZP9Atn4oCnAOvjiCMI6_YkzZNqv_v5pVAHwBhf7UMnC-fKedUWPvYdShhNHFin7pX4a-FqhNHvsqSmDQe6F8yl9Cg4ZV8tzNzZg_28HOLCUVrUFuAgKM57n1_PaB_rb47kuogqk25i-KfQAzuQWtfZROP_DtuFFH83ULcniaNU25y3bjNlma3r2VpZAYMKmujIiCO2eaB00JYVPLoFAEMVRKUPvKUEEIkxSufkFIAZ26UmB8xA43Gp53SiDHbGf9esImLgwOGNyFwZealzC4gcFNe3ILg5cwqB5gcMDgBOOUNXuP427fw2vM0gUs8sxtB7_B9oplkZ8xHiWx0r7UAWUS0IlIoRlCFSRpgMl1JtQ5a2x_xsVPFy7ZgaV3xfY1-nPehDhaZdem5T8BypwQzA |
| link.rule.ids | 786 |
| linkProvider | FAO Food and Agriculture Organization of the United Nations |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Construction+and+verification+of+double+T-DNA+plant+expression+vector+of+the+LFY+gene&rft.jtitle=Molecular+plant+breeding&rft.au=Sun+Lei%2C+Zhang+Qixiang&rft.date=2006-03-01&rft.issn=1672-416X&rft.volume=14&rft.issue=2&rft.externalDocID=Cn2007002034 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1672-416X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1672-416X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1672-416X&client=summon |