Effect of explants and hormones on the adventitious buds differentiation in tissue culture of 'Rieger Begonia'

以丽格海棠的茎段、叶片及叶柄为外植体,通过培养直接诱导不定芽的形成,研究了外植体类型及外植体前处理,生长素、细胞分裂素类型及其配比对不定芽分化的影响。结果表明:丽格海棠中度成熟茎段经4℃冷藏处理后离体培养,死亡率低、分化出芽快、分化率高;叶片及叶柄分化所需时间长、无效芽数多;NAA和6-BA诱导不定芽分化效果好,且6-BA0 75mg/L+NAA0 1mg/L配比中不定芽分化率最高。...

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Bibliographic Details
Published inHebei nong ye da xue xue bao Vol. 28; no. 3
Main Author Han Chao, Fang Zheng
Format Journal Article
LanguageChinese
Published 01.05.2005
Subjects
BEGONIA HIEMALIS
CULTIVO DE TEJIDOS
CULTURE DE TISSU
EXPLANT
EXPLANTES
EXPLANTS
HORMONAS
HORMONE
HORMONES
http://www.fao.org/aos/agrovoc#c_27520
http://www.fao.org/aos/agrovoc#c_32078
http://www.fao.org/aos/agrovoc#c_3663
http://www.fao.org/aos/agrovoc#c_7789
TISSUE CULTURE
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Summary:以丽格海棠的茎段、叶片及叶柄为外植体,通过培养直接诱导不定芽的形成,研究了外植体类型及外植体前处理,生长素、细胞分裂素类型及其配比对不定芽分化的影响。结果表明:丽格海棠中度成熟茎段经4℃冷藏处理后离体培养,死亡率低、分化出芽快、分化率高;叶片及叶柄分化所需时间长、无效芽数多;NAA和6-BA诱导不定芽分化效果好,且6-BA0 75mg/L+NAA0 1mg/L配比中不定芽分化率最高。
Bibliography:F62
2006000772
ISSN:1000-1573