preliminary study of regeneration system of wheat mature embryo and its particle biolistic transformation

以小麦品种晋麦2148的成熟胚为外植体,消毒后用解剖刀刨碎,接种于MS+VB5+11.2g・L-1葡萄糖+2mg・L-12,4D+8g・L-1琼脂糖的培养基中诱导愈伤组织,将诱导3周的成熟胚愈伤组织接种到胚状体成熟培养基(MS+VB5+11.2g・L-1葡萄糖+0.2mg・L-1IAA+8g・L-1琼脂糖)中培养4-8周,然后转接到再生培养基(1/2MS+VB5+30g・L-1蔗糖+8g・L-1琼脂糖)中进行再生成苗.接种1200块成熟胚,得到1123块愈伤组织,最终形成258株再生苗,再生率为21.5%.在建立了再生体系的基础上,用基因枪介导法将GUS基因导入成熟胚愈伤组织,Xgluc染色表...

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Bibliographic Details
Published inJournal of Fujian Agriculture and Forestry University Vol. 34; no. 2
Main Author Lin Deshu, Wang Yanli, Zhuang Zhenhong
Format Journal Article
LanguageChinese
Published 01.06.2005
Subjects
BLE
GENETIC TRANSFORMATION
http://www.fao.org/aos/agrovoc#c_3220
http://www.fao.org/aos/agrovoc#c_32532
http://www.fao.org/aos/agrovoc#c_8373
REBROTE
REGROWTH
REPOUSSE
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
TRIGO
WHEATS
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Summary:以小麦品种晋麦2148的成熟胚为外植体,消毒后用解剖刀刨碎,接种于MS+VB5+11.2g・L-1葡萄糖+2mg・L-12,4D+8g・L-1琼脂糖的培养基中诱导愈伤组织,将诱导3周的成熟胚愈伤组织接种到胚状体成熟培养基(MS+VB5+11.2g・L-1葡萄糖+0.2mg・L-1IAA+8g・L-1琼脂糖)中培养4-8周,然后转接到再生培养基(1/2MS+VB5+30g・L-1蔗糖+8g・L-1琼脂糖)中进行再生成苗.接种1200块成熟胚,得到1123块愈伤组织,最终形成258株再生苗,再生率为21.5%.在建立了再生体系的基础上,用基因枪介导法将GUS基因导入成熟胚愈伤组织,Xgluc染色表明,GUS基因已经在小麦成熟胚愈伤组织中表达.将100块愈伤组织用Xgluc染色,29块愈伤组织出现肉眼可见的蓝色小点.
Bibliography:F02
2006000420
ISSN:1671-5470