The diagnosis of Fusarium culmorum by polyclonal antibodies - preparation and character of antigens and antibodies

Eighteen monospore Fusarium culmorum (FCu) isolates with different pathogenicity were cultivated on five liquid media. The composition of media influenced morphological and physiological characters of isolates. Antigens were prepared by extraction of proteins from mycelial mats in a special buffer,...

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Published inOchrana Rostlin - UZPI (Czech Republic) Vol. 33; no. 2
Main Authors Kratka, J, Kynerova, B, Zemanova, A, Sykorova, S. (Vyzkumny Ustav Rostlinne Vyroby, Prague-Ruzyne (Czech Republic). Odbor Rostlinolekarstvi)
Format Journal Article
LanguageEnglish
Published 01.03.1997
Subjects
ANTIGENE
ANTIGENOS
DIAGNOSTIC
DIAGNOSTICO
ELECTROFORESIS
ELECTROPHORESE
FUSARIUM CULMORUM
MEDIO DE CULTIVO
MICELIO
MILIEU DE CULTURE
MYCELIUM
PROTEINAS
PROTEINE
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Summary:Eighteen monospore Fusarium culmorum (FCu) isolates with different pathogenicity were cultivated on five liquid media. The composition of media influenced morphological and physiological characters of isolates. Antigens were prepared by extraction of proteins from mycelial mats in a special buffer, then purified by centrifugation and ultracentrifugation of supernatant. The composition of media influenced quantity and quality of proteins of antigens. The pathogenicity of FCu isolates was not correlated with protein content of antigens.
Bibliography:U30
H20
9700793
ISSN:0862-8645