Determination of ochratoxin A in beer

• Published in: Czech Journal of Food Sciences Vol. 23; no. 2
• Main Authors: Dasko, L.(Vyskumny Ustav Potravinarsky, Bratislava (Slovak Republic))E-mail:dasko@vup.sk, Belajova, E, Rauova, D, Kovac, M
• Format: Journal Article
• Language: English
• Subjects: ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; BEERS; BIERE; BIOLOGICAL CONTAMINATION; CERVEZAS; CONTAMINACION BIOLOGICA; CONTAMINATION BIOLOGIQUE; FLUORESCENCE; FLUORESCENCIA; HPLC; LIMITE MAXIMALE DE RESIDU; LIMITES MAXIMOS DE RESIDUOS; MAXIMUM RESIDUE LIMITS; METHODE; METHODS; METODOS; MONOCLONAL ANTIBODIES; OCHRATOXINE; OCHRATOXINS; OCRATOXINAS; SEPARACION; SEPARATING; SEPARATION
• ISSN: 1212-1800

The aim of this study was to test the use of immunoaffinity columns and the HPLC method coupled with a fluorescent detector in order to estimate ochratoxin A (OTA) concentrations in beer. Twenty beer samples with different ethanol concentrations from Slovak and 4 beer samples from foreign breweries were used in the study. Sample extracts were purified on Ochraprep immunoaffinity columns with a monoclonal antibody to bind OTA. This pre-treatment procedure offered a very sensitive detection level (10,000 times lower than the maximum residue limit). Experiments with artificially contaminated beers led to good results of HPLC performance characteristics (detection limit, repeatability, calibration, recovery, uncertainty). No positive results in any of the 24 beer samples tested were found. The method used is suitable for the determination of OTA concentrations in beer, its detection limit being 0.00075 microg/L.