Determination of fumonisins B1 and B2 in beer

• Published in: Czech Journal of Food Sciences Vol. 23; no. 1
• Main Authors: Dasko, L.(Vyskumny Ustav Potravinarsky, Bratislava (Slovak Republic))E-mail:dasko@vup.sk, Rauova, D, Belajova, E, Kovac, M
• Format: Journal Article
• Language: English
• Subjects: BEERS; BIERE; BIOLOGICAL CONTAMINATION; CERVEZAS; CONTAMINACION BIOLOGICA; CONTAMINATION BIOLOGIQUE; FLUORESCENCE; FLUORESCENCIA; FUMONISINAS; FUMONISINE; FUMONISINS; HPLC; METHODE; METHODS; METODOS; SEPARACION; SEPARATING; SEPARATION
• ISSN: 1212-1800

A total of 20 Slovak beers produced from the 2003 barley harvest by different companies were analysed for fumonisin B1 and B2 contamination. A method based on immunoaffinity column extraction and clean-up, coupled with HPLC analysis with fluorescence detection after derivatisation with a mixture of o-phthaldialdehyde and 2-mercaptoethanol, was used. The detection limit for both fumonisins was 1 microg/mL, the recovery was 93% for fumonisin B1 and 78% for fumonisin B2. Phosphate buffer usually applied resulted in a poor separation of derivatised fumonisins. Peak splitting depended on the pH of the eluent. The pH value of 2.6 was found suitable for the peak splitting elimination. A convenient gradient elution metod was suggested to avoid possible interference in the determination of fumonisin concentrations. Fumonisins concentrations were under the limit of detection in all the beer samples tested.