Determination of fumonisins B1 and B2 in beer

A total of 20 Slovak beers produced from the 2003 barley harvest by different companies were analysed for fumonisin B1 and B2 contamination. A method based on immunoaffinity column extraction and clean-up, coupled with HPLC analysis with fluorescence detection after derivatisation with a mixture of...

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Bibliographic Details
Published inCzech Journal of Food Sciences Vol. 23; no. 1
Main Authors Dasko, L.(Vyskumny Ustav Potravinarsky, Bratislava (Slovak Republic))E-mail:dasko@vup.sk, Rauova, D, Belajova, E, Kovac, M
Format Journal Article
LanguageEnglish
Subjects
BEERS
BIERE
BIOLOGICAL CONTAMINATION
CERVEZAS
CONTAMINACION BIOLOGICA
CONTAMINATION BIOLOGIQUE
FLUORESCENCE
FLUORESCENCIA
FUMONISINAS
FUMONISINE
FUMONISINS
HPLC
METHODE
METHODS
METODOS
SEPARACION
SEPARATING
SEPARATION
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Summary:A total of 20 Slovak beers produced from the 2003 barley harvest by different companies were analysed for fumonisin B1 and B2 contamination. A method based on immunoaffinity column extraction and clean-up, coupled with HPLC analysis with fluorescence detection after derivatisation with a mixture of o-phthaldialdehyde and 2-mercaptoethanol, was used. The detection limit for both fumonisins was 1 microg/mL, the recovery was 93% for fumonisin B1 and 78% for fumonisin B2. Phosphate buffer usually applied resulted in a poor separation of derivatised fumonisins. Peak splitting depended on the pH of the eluent. The pH value of 2.6 was found suitable for the peak splitting elimination. A convenient gradient elution metod was suggested to avoid possible interference in the determination of fumonisin concentrations. Fumonisins concentrations were under the limit of detection in all the beer samples tested.
Bibliography:http://www.cazv.cz
2006000226
U30
Q03
http://www.cazv.cz/attachments/3-Dasko.pdf
ISSN:1212-1800