Relationships between some hordein components and quality properties in two Tunisian barley varieties as influenced by nitrogen fertilisation

• Published in: Czech Journal of Genetics and Plant Breeding Vol. 41; no. 1
• Main Authors: Bettaieb-Ben Kaab, L.(Faculty of Science of Tunis, El Manar (Tunisia). Dept. of Biology)E-mail:leila.bk@planet.tn, El Felah, M.(INRAT, Tunisia (Tunisia). Dept. of Genetics), Lauriere, M.(INRA, Thiverval-Grignon (France). Centre of Biotechnology Agro-Industry)
• Format: Journal Article
• Language: English
• Subjects: ABONOS NITROGENADOS; ALBUMINAS; ALBUMINE; ALBUMINS; APLICACION DE ABONOS; BARLEY; CEBADA; CHEMICAL COMPOSITION; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; CONTENIDO DE NITROGENO; ELECTROFORESIS; ELECTROPHORESE; ELECTROPHORESIS; ENGRAIS AZOTE; FERTILISATION; FERTILIZER APPLICATION; GRAIN; GRANOS; HORDEUM VULGARE; NITROGEN CONTENT; NITROGEN FERTILIZERS; ORGE; PESO; POIDS; PROLAMINAS; PROLAMINE; PROLAMINES; SEPARACION; SEPARATING; SEPARATION; TENEUR EN AZOTE; VARIEDADES; VARIETE; VARIETIES; WEIGHT
• ISSN: 1212-1975; 1212-1975

Two Tunisian barley varieties, Martin and Rihane, differing by their degrees of adaptation to the environmental conditions and grain yield potential, were grown under different levels of nitrogen fertilisation. The effect of N supply on the hordein components (expressed in mg per albumen) was determined by Nu-PAGE electrophoresis using the MOBS running buffer and densitometric analysis. Increasing N fertilisation increased the protein content by increasing the amount of C-hordeins. The sub-fraction BIII of high molecular weight B-hordeins appeared as the best criterion for distinguishing between the two barley varieties, and its quantification could be recommended for the understanding of the quality properties of the barley response to different environmental conditions, particularly N fertilisation.