Adventitious shoot regeneration and micropropagation in Calendula officinalis L

• Published in: Biologia plantarum Vol. 48; no. 3
• Main Authors: Coecue, S.(University of Ankara (Turkey). Dept. of Field Crops), Uranbey, S.(Ministry of Agriculture and Rural Affairs, Ankara (Turkey). Central Field Crops Research Inst.), Ipek, A.(University of Ankara (Turkey). Dept. of Field Crops), Khawar, K.M.(University of Ankara (Turkey). Dept. of Field Crops), Sarihan, E.O.(University of Ankara (Turkey). Dept. of Field Crops), Kaya, M.D.(University of Ankara (Turkey). Dept. of Field Crops), Parmaksiz, I.(University of Ankara (Turkey). Dept. of Field Crops), Oezcan, S.(University of Ankara (Turkey). Dept. of Field Crops)E-mail:ozcan@agri.ankara.edu.tr
• Format: Journal Article
• Language: English
• Subjects: AIB; ANA; BROTES; CALENDULA; CLONACION; CLONAGE; CLONING; COTILEDONES; COTYLEDON; COTYLEDONS; CULTIVO IN VITRO; CULTURE IN VITRO; DRUG PLANTS; EXPLANT; EXPLANTES; EXPLANTS; HIPOCOTILOS; HYPOCOTYLE; HYPOCOTYLS; IBA; IN VITRO CULTURE; IN VITRO REGENERATION; KINETIN; KINETINE; LARGURA; LENGTH; LONGUEUR; MICROPROPAGACION; MICROPROPAGATION; MONOCARPOS ORNAMENTALES; MULTIPLICATION DES PLANTES; NAA; ORGANOGENESE; ORGANOGENESIS; ORNAMENTAL MONOCARPS; PLANT PROPAGATION; PLANTAS MEDICINALES; PLANTE MEDICINALE; PLANTE ORNEMENTALE MONOCARPIQUE; POUSSE; PROPAGACION DE PLANTAS; QUINETINA; REGENERACION IN VITRO; REGENERATION IN VITRO; SHOOTS; THIDIAZURON; TIDIAZURON
• ISSN: 0006-3134; 1573-8264

Hypocotyl, cotyledon and cotyledonary node explants of Calendula officinalis were cultured on Murashige and Skoog media supplemented with various concentrations of thidiazuron (TDZ), kinetin (KIN), alpha-naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) to induce adventitious shoot regeneration and micropropagation. The highest frequency of adventitious shoot regeneration was achieved from hypocotyl and cotyledon explants on MS media supplemented with 0.75 mg/cubic dm TDZ and either 0.25 or 0.50 mg/cubic dm IBA. Efficient in vitro clonal propagation was also induced from cotyledonary nodes on a range of media supplemented with 0.75 mg/cubic dm TDZ and 0.05 mg/cubic dm NAA or 2 mg/cubic dm KIN and 1 mg/cubic dm NAA. Regenerated shoots were excised and rooted in MS medium supplemented with 1 mg/cubic dm NAA. The rooted plantlets were finally transferred to pots.