Effect of Fusarium head blight on mycotoxin content in grain of spring barley cultivars

• Published in: Czech Journal of Genetics and Plant Breeding Vol. 40; no. 3
• Main Authors: Sip, V.(Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic))E-mail:sip@vurv.cz, Tvaruzek, L.(Zemedelsky Vyzkumny Ustav, Kromeriz (Czech Republic)), Chrpova, J.(Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic)), Sykorova, S.(Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic)), Leisova, L.(Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic)), Kucera, L.(Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic)), Ovesna, J.(Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic))
• Format: Journal Article
• Language: English
• Subjects: ADN; CONTROL DE ENFERMEDADES; CONTROLE DE MALADIES; CULTIVOS DE PRIMAVERA; CULTURE DE PRINTEMPS; DISEASE CONTROL; DISEASE RESISTANCE; DNA; EXPERIMENTACION EN CAMPO; EXPERIMENTAL INFECTION; EXPERIMENTATION AU CHAMP; FIELD EXPERIMENTATION; FONGICIDE; FUNGICIDAS; FUNGICIDES; FUSARIUM CULMORUM; GIBBERELLA ZEAE; GRAIN; GRANOS; HORDEUM VULGARE; INFECCION EXPERIMENTAL; INFECTION EXPERIMENTALE; PCR; PESO; POIDS; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; SPRING CROPS; VARIEDADES; VARIETE; VARIETIES; VOMITOXIN; VOMITOXINA; VOMITOXINE; WEIGHT; ZEARALENONA; ZEARALENONE
• ISSN: 1212-1975; 1212-1975

Field infection experiments with six spring barley cultivars registered in the Czech Republic and resistance sources Chevron and CI 4196 were carried out. One of the four Fusarium isolates used for inoculations was a predominant nivalenol producer, while the other isolates were deoxynivalenol (DON) producers. Out of the other mycotoxins 3-AcDON was found in grain at a relatively high concentration. Significant cultivar differences in DON content, examined yield traits, percentage of Fusarium colonies and percentage of non-germinating seeds were detected after inoculum spraying on two dates and mist irrigation of infected plots. When inoculated with aggressive isolate of F. culmorum Chevron and CI 4196 cvs. showed high resistance and the cultivars Jersey, Olbram and Scarlett moderate resistance to DON accumulation in grain. Treatment with fungicide (active ingredient tebuconazole) led on average to a 52.5% reduction of DON content, but the efficacy of fungicide treatment was highly influenced by year and cultivar. DON concentration was closely related only with the transformed threshold cycle values for Fusarium target amplicons from quantitative real time PCR analysis.