Ploidy identification of doubled chromosome number plants in Viola x wittrockiana M 1-generation

The aim of this study was to develop a protocol for production of polyploid M1-generation plants of Viola x wittrockiana. Two variants of colchicine treatment were compared for their efficiency. Early detection of novel ploidy levels was achieved by screening of stomata size, leaf index value (leaf...

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Published inHorticultural science (Praha) Vol. 29; no. 1
Main Authors Ajalin, I, Kobza, F. (Mendelova Zemedelska a Lesnicka Univ., Lednice na Morave (Czech Republic). Zahradnicka Fakulta), Dolezel, J
Format Journal Article
LanguageEnglish
Published 01.03.2002
Subjects
ANATOMIA DE LA PLANTA
ANATOMIE VEGETALE
CELL STRUCTURE
COLCHICINA
COLCHICINE
CZECH REPUBLIC
ESTRUCTURA CELULAR
GENETICA MOLECULAR
GENETIQUE MOLECULAIRE
HEXAPLOIDIA
HEXAPLOIDIE
HEXAPLOIDY
MOLECULAR GENETICS
PLANT ANATOMY
REPUBLICA CHECA
REPUBLIQUE TCHEQUE
STRUCTURE CELLULAIRE
VIOLA
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Summary:The aim of this study was to develop a protocol for production of polyploid M1-generation plants of Viola x wittrockiana. Two variants of colchicine treatment were compared for their efficiency. Early detection of novel ploidy levels was achieved by screening of stomata size, leaf index value (leaf blade length/width), and other morphological characteristics of the M1-generation. Secondary screening for novel ploidy levels was performed by flow cytometry (FCM). Hexadecaploid, aneuploid, and mixoploid plants were successfully identified by FCM.
Bibliography:2002000452
F30
ISSN:0862-867X