The effect of nutrient medium on growth of Escherichia coli bacteria with pBI 121 plasmid DNA

The effect of nutrient medium on growth of Escherichia coli containing the 13 kbp pBI 121 plasmid and on yield of isolated plasmid DNA were examined. Six nutrient media (LB, SOB, SOC, King B, MPA(g) and YDC) were tested in five repetitions. The highest bacterial biomass after 290 min of cultivation...

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Published inPlant Protection Science - UZPI (Czech Republic) Vol. 34; no. 2
Main Authors Mraz, I, Petrzik, K, Sip, M. (Akademie Ved, Ceske Budejovice (Czech Republic). Ustav Molekularni Biologie Rostlin)
Format Journal Article
LanguageEnglish
Published 01.07.1998
Subjects
ADN
CULTURE MEDIA
DNA
ESCHERICHIA COLI
EXTRACTOS DE CARNE
EXTRACTOS DE LEVADURA
EXTRAIT DE LEVURE
EXTRAIT DE VIANDE
FOSFATOS
GLUCOSA
GLUCOSE
GROWTH RATE
INDICE DE CRECIMIENTO
MAGNESIO
MAGNESIUM
MEAT EXTRACTS
MEDIO DE CULTIVO
MILIEU DE CULTURE
PHOSPHATE
PHOSPHATES
PLASMIDE
PLASMIDIOS
PLASMIDS
TAUX DE CROISSANCE
TEMPS
TIEMPO
TIME
YEAST EXTRACTS
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Summary:The effect of nutrient medium on growth of Escherichia coli containing the 13 kbp pBI 121 plasmid and on yield of isolated plasmid DNA were examined. Six nutrient media (LB, SOB, SOC, King B, MPA(g) and YDC) were tested in five repetitions. The highest bacterial biomass after 290 min of cultivation was observed in the media LB (OD sub(550) 0.745), King B (OD sub(550) 0.647) and SOB (OD sub(550) 0.553). The highest yield of plasmid DNA was obtained from E. coli cultivated in MPA(g) medium (305 ng/ml). The yields from King B (150 ng/ml), SOC (113 ng/ml) and SOB (84 ng/ml) were also higher than the yield from LB medium (47 ng/ml). We conclude that the best media to culture E. coli with large plasmids such as pBI 121 are those enriched with meat extract and glucose (MPA(g)) or nutrient media enriched with Mg2+ and PO43- ions (King B).
Bibliography:U30
Q03
1998000919
ISSN:0862-8645