Enzyme-amplified ELISA for detection of beet mild yellowing virus in aphids

Two serological tests, double antibody sandwich ELISA (DAS-ELISA) and enzyme-amplified ELISA (AMP-ELISA) were used to detect beet mild yellowing virus (BMYV) in aphids (Myzus persicae). AMP-ELISA appeared to be more sensitive and reliable for detection. Absorbance values of AMP-ELISA were approximat...

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Bibliographic Details
Published inPlant Protection Science - UZPI (Czech Republic) Vol. 34; no. 2
Main Author Polak, J. (Vyzkumny Ustav Rostlinne Vyroby, Prague-Ruzyne (Czech Republic))
Format Journal Article
LanguageEnglish
Published 01.07.1998
Subjects
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
ELISA
MYZUS PERSICAE
PLANT VIRUSES
TEST ELISA
VIRUS DE LAS PLANTAS
VIRUS DES VEGETAUX
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Summary:Two serological tests, double antibody sandwich ELISA (DAS-ELISA) and enzyme-amplified ELISA (AMP-ELISA) were used to detect beet mild yellowing virus (BMYV) in aphids (Myzus persicae). AMP-ELISA appeared to be more sensitive and reliable for detection. Absorbance values of AMP-ELISA were approximately twice those with standard ELISA. Reliability of BMYV detection was higher after 48 h of acquisition time than with 24 h acquisition. BMYV was reliably detected in samples prepared from groups of four and more aphids. BMYV was not detected in individual aphids after 24 h of acquisition, but two out of five aphids were found to be BMYV positive after 48 h of acquisition. While AMP-ELISA is a more suitable method for detection of BMYV in aphids, low virus concentrations in aphids make a reliable detection of BMYV in individual aphids impossible.
Bibliography:U30
H10
H20
1998000915
ISSN:0862-8645