Study on doubling efficiency of maize haploids bud soaked by herbicide and colchicine

• Published in: Jilin nongye daxue xuebao Vol. 35; no. 4
• Main Authors: Wang He, Jilin Agricultural University, Changchun (China), College of Agronomy, Li Jizhu, Jilin Agricultural University, Changchun (China), College of Agronomy, Zhang Jiwei, High School attached to Northeast Normal University, Changchun (China)
• Format: Journal Article
• Language: Chinese
• Published: 01.08.2013
• Subjects: GRAINE; http://www.fao.org/aos/agrovoc#c_12332; http://www.fao.org/aos/agrovoc#c_5993; http://www.fao.org/aos/agrovoc#c_6946; MAIS; MAIZ; MAIZE; PLANTE; PLANTS; SEEDS; SEMILLA
• ISSN: 1000-5684

以Mo17×PHB1M诱导的单倍体子粒为试验材料，利用戊炔草胺、甲基胺草磷和秋水仙素为加倍药剂，浸芽处理单倍体种子。结果表明：戊炔草胺质量浓度为4-16 mg/L时的加倍率为5%-16%；甲基胺草磷质量浓度为8-32mg/L时的加倍率为8%-16%；秋水仙素质量浓度为0.4-1.0mg/L时的加倍率为5.5%-16.5%，3种药剂对玉米单倍体加倍均有效果。其中8mg/L的戊炔草胺、21mg/L的甲基胺草磷、0.7mg/L的秋水仙素浸芽8h的玉米单倍体加倍率均为各组最高。戊炔草胺和甲基胺草磷可以替代秋水仙素加倍玉米单倍体。 Maize haploids used in this research were induced from Mo17 × PHB1M. Haploids by bud soaking. The results showed all three medicaments had effects on doubling haploids. The doubling rates of haploids treated with propyzamide of 4 - 16 mg/L were 5% - 16%. The doubling rates of haploids treated with AMP of 8 - 32 mg/L were 8% - 16%. The doubling rates of haploids treated with AMP of 0.4 - 1.0