Identification of vegetable root-knot nematode from Gansu province and its rDNA-ITS sequence analysis

• Published in: Zhiwu baohu Vol. 39; no. 1
• Main Authors: Du Hui, Gansu Academy of Agricultural Sciences, Lanzhou (China), Institute of Plant Protection, Qi Yonghong, Gansu Academy of Agricultural Sciences, Lanzhou (China), Institute of Plant Protection, Lu Heping, Gansu Academy of Agricultural Sciences, Lanzhou (China), Institute of Plant Protection
• Format: Journal Article
• Language: Chinese
• Published: 01.02.2013
• Subjects: GENE; GENES; HORTALIZAS; http://www.fao.org/aos/agrovoc#c_3214; http://www.fao.org/aos/agrovoc#c_5993; http://www.fao.org/aos/agrovoc#c_8174; LEGUME; PLANTE; PLANTS; VEGETABLES
• ISSN: 0529-1542

为明确甘肃省保护地蔬菜根结线虫的种类，采用rDNA-ITS-PCR方法结合其2龄幼虫、雌虫会阴花纹等形态学特征对采自甘肃省9个市的主要蔬菜生产基地保护地蔬菜根结线虫的11个种群样本进行了种类鉴定，并对其ITS区域进行序列测定及分析比对。结果表明11个根结线虫种群的ITS区域序列与GenBank数据库已知南方根结线虫（Meloidogyne incognita）ITS区域序列的相似性达到99%，其ITS片段大小介于690-695bp之间，表明甘肃省保护地中蔬菜根结线虫为南方根结线虫。 To clear the species of vegetable root-knot nematodes in Gansu Province, 11 root-knot nematode (Meloidogyne sp.) populations collected from greenhouse vegetables in Gansu Province were identified based on rDNA-ITS sequence analysis and the perineal pattern of female nematodes. The results indicated that similarity between rDNA-ITS sequences of 11 root-knot nematode samples and M. incognita species from GenBank database reached 99%