Cloning and expression analysis of a disease resistant gene VaAC1 in adzuki bean

• Published in: Nanjing nongye daxue xuebao Vol. 36; no. 1
• Main Authors: Chen Xin, Nanjing Agricultural University,Nanjing(China),College of Agriculture, Chen Huatao, Jiangsu Academy of Agricultural Sciences,Nanjing（China），Institute of Vegetable Crops, Wan Jianmin, Nanjing Agricultural University,Nanjing(China),College of Agriculture
• Format: Journal Article
• Language: Chinese
• Published: 01.01.2013
• Subjects: ADZUKI BEANS; AMELIORATION DES PLANTES; BIOTECHNOLOGIE; BIOTECHNOLOGY; BIOTECNOLOGIA; CLONACION; CLONAGE; CLONING; EXPRESION GENICA; EXPRESSION DES GENES; FITOMEJORAMIENTO; FRIJOL ADZUKI; GENE EXPRESSION; HARICOT ADZUKI; http://www.fao.org/aos/agrovoc#c_15952; http://www.fao.org/aos/agrovoc#c_16165; http://www.fao.org/aos/agrovoc#c_27527; http://www.fao.org/aos/agrovoc#c_5956; http://www.fao.org/aos/agrovoc#c_8177; http://www.fao.org/aos/agrovoc#c_8632; MULTIPLICATION VEGETATIVE; PLANT BREEDING; PROPAGACION VEGETATIVA; VEGETATIVE PROPAGATION

利用简并引物从小豆中扩增出1条与植物几丁质酶基因高度同源的片段,命名为VaAC1,通过RACE技术延伸VaAC1基因的3＇端和5＇端,VaAC1基因完整ORF长度为885 bp,编码294个氨基酸,拥有几丁质酶蛋白家族典型保守结构域GH18_chitinase-like。RT-PCRåˆ...