Cloning and expression analysis of a disease resistant gene VaAC1 in adzuki bean
å©ç¨ç®å¹¶å¼ç©ä»å°è±ä¸æ©å¢åº1æ¡ä¸æ¤ç©å ä¸è´¨é ¶åºå é«åº¦åæºçç段,å½å为VaAC1,éè¿RACEææ¯å»¶ä¼¸VaAC1åºå ç3ï¼ç«¯å5ï¼ç«¯,VaAC1åºå å®æ´ORFé¿åº¦ä¸º885 bp,ç¼ç 294个氨åºé ¸,æ¥æå ä¸è´¨é ¶èç½å®¶æå ¸åä¿å®ç»æåGH18_chitinase-likeãRT-PCRå...
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Published in | Nanjing nongye daxue xuebao Vol. 36; no. 1 |
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Main Authors | , , |
Format | Journal Article |
Language | Chinese |
Published |
01.01.2013
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Subjects | |
Online Access | Get more information |
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Summary: | å©ç¨ç®å¹¶å¼ç©ä»å°è±ä¸æ©å¢åº1æ¡ä¸æ¤ç©å ä¸è´¨é
¶åºå é«åº¦åæºçç段,å½å为VaAC1,éè¿RACEææ¯å»¶ä¼¸VaAC1åºå ç3ï¼ç«¯å5ï¼ç«¯,VaAC1åºå å®æ´ORFé¿åº¦ä¸º885 bp,ç¼ç 294个氨åºé
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¸åä¿å®ç»æåGH18_chitinase-likeãRT-PCRåæ表æ,VaAC1åºå å¨å°è±æ ¹ãèåå¶ä¸åæ表达,ä¸å¨å¶ä¸ç表达ç¸å¯¹è¾é«;大è±è±å¶ç
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In this study,a chitinase homologue gene was cloned using degenerate primers in adzuki bean,and the gene was named VaAC1.The 3â² end and 5â² end sequences of VaAC1 gene were extended by RACE method.As a result,the complete ORF of VaAC1 gene was 885 bp,encoding 294 amino acids.VaAC1 had a typical conserved domain GH18_chitinase-like of the chitinase protein family.Expressions of VaAC1 gene were detected in root,stem and leaf using RT-PCR method in adzuki bean,and the expression of VaAC1 gene in lea |
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Bibliography: | F30 2014000952 |
ISSN: | 1000-2030 |