Study on the method of detecting phytase activity of corn grains

对转植酸酶基因玉米子粒中植酸酶活性检测方法中的影响因素进行了研究和优化。正交试验结果表明：底物浓度0.1 mmol/L, pH 5.5, 反应时间30 min为最佳测定条件。 Orthogonal test method is used to study the best detection method of the phytase from transgenic corn grains. It was found that the optimum detection conditions are pH 5.5 buffer liquid at the concentration of su...

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Bibliographic Details
Published inJilin nongye daxue xuebao Vol. 34; no. 1
Main Authors Liu Na, Jilin Academy of Agricultural Sciences, Changchun (China), Biotechnology Research Center, Li Jing, Jilin University, Changchun (China), School of Public Health, Liu Hongmin, Jilin University, Changchun (China), School of Public Health
Format Journal Article
LanguageChinese
Published 01.02.2012
Subjects
FITASA
GRAIN
GRANOS
http://www.fao.org/aos/agrovoc#c_12332
http://www.fao.org/aos/agrovoc#c_3346
http://www.fao.org/aos/agrovoc#c_33525
MAIS
MAIZ
MAIZE
PHYTASE
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Summary:对转植酸酶基因玉米子粒中植酸酶活性检测方法中的影响因素进行了研究和优化。正交试验结果表明：底物浓度0.1 mmol/L, pH 5.5, 反应时间30 min为最佳测定条件。 Orthogonal test method is used to study the best detection method of the phytase from transgenic corn grains. It was found that the optimum detection conditions are pH 5.5 buffer liquid at the concentration of substrate of 0.1 mmol/L and reacting for 30 minutes.
Bibliography:F60
2013002984
ISSN:1000-5684