Multiplex PCR assay for detection of wheat leaf rust resistance genes Lr9-Lr26 and Lr19-Lr20 in 116 Chinese wheat cultivars（lines

• Published in: Zhiwu baohu Vol. 38; no. 2
• Main Authors: Ren Xiaoli, Chinese Academy of Agricultural Sciences, Beijing (China), Institute of Plant Protection, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Liu Taiguo, Chinese Academy of Agricultural Sciences, Beijing (China), Institute of Plant Protection, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Liu Bo, Chinese Academy of Agricultural Sciences, Beijing (China), Institute of Plant Protection, State Key Laboratory for Biology of Plant Diseases and Insect Pests
• Format: Journal Article
• Language: Chinese
• Published: 01.04.2012
• Subjects: BLE; GENE; GENES; http://www.fao.org/aos/agrovoc#c_3214; http://www.fao.org/aos/agrovoc#c_34079; http://www.fao.org/aos/agrovoc#c_6710; http://www.fao.org/aos/agrovoc#c_8373; PCR; ROUILLE; ROYA; RUSTS; TRIGO; WHEATS
• ISSN: 0529-1542

[目的]建立简单、快速、有效的小麦抗叶锈基因复合PCR体系, 从而提高分子标记辅助选择效率。[方法]以28个‘Thatcher’为背景的近等基因系和16个已知基因载体品系作为试材, 测试了小麦抗叶锈病基因Lr9、Lr26、Lr19和Lr20的STS标记特异性,通过优化PCR反应体系和循环条件, 构建了抗叶锈基因Lr9-Lr26和Lr19-Lr20的复合PCR检测体系。对116个小麦品种（系）所含有的抗叶锈病基因进行了分子检测。[结果] 供试品种均不含有Lr9和Lr20, 47个品种含有Lr26（基因频率为40.5%）,‘中梁22’含有Lr19。经反复验证, Lr9-Lr26和Lr19-Lr20复合PCR技术检测结果可靠, 且与上述单个分子标记检测结果一致。[结论] 建立的Lr9-Lr26和Lr19-Lr20的复合PCR检测体系可以准确、稳定、高效地检测小麦抗叶锈基因Lr9、Lr26、Lr19和Lr20。 [Objective] A simple, fast and effective method of multiplex PCR for wheat leaf rust resistance genes was set up to improve the efficiency of marker-assisted selection. [Method] By testing the specificity of STS markers of Lr9, Lr19, Lr20 and Lr26 based on the near isogenic lines with＇Thacher＇background and known gene lines, multiplex PCR was developed and optimized for detection of wheat leaf rust resistance gene combination of Lr9-Lr26 and Lr19-Lr20. [Result] A total of 116 wheat cultivars were evaluated using the multiplex PCRs, and specific DNA bands of resistance gene Lr26 was present in 47 accessions ou