Diversity and antimicrobial activity of endophytic fungi in Sophora alopecuroides L. from Baijitan National Nature Reserve of Ningxia

• Published in: Xi bei nong lin ke ji da xue xue bao.Zi ran ke xue ban Vol. 40; no. 5
• Main Authors: Gu Peiwen, Ningxia University, Yinchuan (China), Agricultural College, Hao Li, Ningxia University, Yinchuan (China), Agricultural College, Xu Run, Ningxia University, Yinchuan (China), Agricultural College
• Format: Journal Article
• Language: Chinese
• Published: 01.05.2012
• Subjects: http://www.fao.org/aos/agrovoc#c_16141; http://www.fao.org/aos/agrovoc#c_16292; http://www.fao.org/aos/agrovoc#c_5184; NATURE RESERVES; NINGXIA (HUI); RESERVAS NATURALES; RESERVE NATURELLE; SOPHORA
• ISSN: 1671-9387

探索宁夏干旱荒漠区苦豆子内生真菌的区系组成及特点，为苦豆子内生真菌的合理开发和利用提供理论依据。从宁夏白芨滩国家级自然保护区植被、土壤类型等不同的5个样区采集苦豆子样品25份，分离苦豆子内生真菌，并根据培养性状、菌落、孢子等形态特征对其进行鉴定；根据苦豆子的侵入率、分离率、物种多样性指数和相似性系数，分析其区系组成特点；采用琼脂移块法，检测所分离的内生真菌对番茄早疫病菌（Alternaria solani）、小麦赤霉病菌（Fusarium graminearum）、番茄灰霉病菌（Botrytis cinerea）、黄瓜枯萎病菌（Fusarium oxysporum）、马铃薯干腐病菌（Fusarium sulphureum）、辣椒炭疽病菌（Colletotrichum capsici）等6株植物病原菌的的抑菌活性。从25份苦豆子样品中，共分离到214株内生真菌。同一植株不同组织中的内生真菌数量以种子最多，叶部最少；植被类型中，沙生植被草原比荒漠草原分离的苦豆子内生真菌多。经鉴定，从各样区分离的苦豆子内生真菌分属22个属，其中卵形孢霉属（Oospora）和头孢霉属（Cephalosporium）为样区共有属。样区Ⅰ与样区Ⅲ及样区Ⅳ与样区Ⅴ的内生真菌群落有密切的亲缘关系，相似性较高。214株内生真菌中有167株（78.0%）有抑菌活性，不同属内生真菌的抑菌率差异较大。宁夏干旱荒漠区苦豆子内生真菌具有丰富的多样性和较高的抑菌生物活性。 The composition, diversity and antimicrobial activity of endophytic fungi from Sophora alopecuroides L. in arid and desolate areas of Ningxia were analyzed, which could also provide research methods and theory on rational development and use of endophytic fungi from S. alopecuroides L. The endophytic fungi were separated and cultivated from 25 samples of healthy seeds and plants of S. alopecuroide