Inhibitory effect of four kinds of Chinese herbs and triple compounds against main pathogenic bacteria from cultivated eels

• Published in: Huazhong nongye daxue xuebao Vol. 30; no. 6
• Main Authors: Guan Ruizhang, Jimei University, Xiamen(China), Fishery College, Li Zhongqin, Jimei University, Xiamen(China), Fishery College, Guo Songlin, Jimei University, Xiamen(China), Fishery College
• Format: Journal Article
• Language: Chinese
• Published: 01.12.2011
• Subjects: AGENT PATHOGENE; ANGUILA; ANGUILLE; EELS; http://www.fao.org/aos/agrovoc#c_2490; http://www.fao.org/aos/agrovoc#c_28427; http://www.fao.org/aos/agrovoc#c_5630; INHIBICION; INHIBITION; ORGANISMOS PATOGENOS; PATHOGENS
• ISSN: 1000-2421

摘　　要：应用超微粉碎法将虎杖、石榴皮、大黄以及五倍子4种中药进行加工处理至粒径为5～10μm后,组成4个三联用的中药复方,针对近年来从福建各地养殖场病鳗中常检测到的9株致病菌,采用琼脂稀释法测定其抑菌最低浓度。试验结果表明：虎杖、石榴皮、大黄和五倍子组合的4个中药复方对9株养殖鳗鲡主要致病菌均有一定的协同抑制作用,其协同效应因子FIC范围在0.38～0.76。其中,联用效果最好的是复方1,所联用的3种中药对9株致病菌均具有显著协同抗菌效应,FIC≤0.5。每个复方的联用3种中药的最低抑菌浓度均比单用浓度降低了79%以上,不但可大大降低用药量,而且用药成本比单用节省47%以上。 Rhus chinensis Mill（RCM）,Rheum palmatum Linn（RPL）,Rhizoma polygoni Cuspidati（RPC）,and Punica granatum Linn（PGL）,were first ground into 5-10 μm superfine powder with ultra-pulverization.Then they were mixed one by one based on chessboard experimental method to get four kinds of compounds of Chinese herbs.To study inhibitation activity of the compounds,the minimum inhibitory concentration（MIC） and the minimum bactericidal concentration（MBC） of single drug and the triple compounds against nine strains of pathogenic bacteria from eels were measured with agar dilution method.The results showed that four kinds of Chinese herbs had certain antibacterial effects on nine bacteria.RCM had the best antibacterial effect,followed by P