Multiplex PCR detection of wheat rust fungi

• Published in: Henan nongye daxue xuebao Vol. 44; no. 1
• Main Authors: Meng Haoguang, Henan Agricultural University,Zhengzhou(China), College of Plant Protection, Cao Lihua, Henan Agricultural University,Zhengzhou(China), College of Plant Protection, Zong Xianzhao, Henan Agricultural University,Zhengzhou(China), College of Plant Protection
• Format: Journal Article
• Language: Chinese
• Published: 01.01.2010
• Subjects: BLE; http://www.fao.org/aos/agrovoc#c_34079; http://www.fao.org/aos/agrovoc#c_6710; http://www.fao.org/aos/agrovoc#c_8373; PCR; ROUILLE; ROYA; RUSTS; TRIGO; WHEATS

利用真菌β-微管蛋白基因的保守序列和小麦条锈菌（Puccinia striiformis f.sp.tritici）、叶锈菌（Puccinia tri-ticina）的SCAR标记引物,对锈菌孢子与感病小麦叶片总DNA进行复合PCR检测,扩增获得了小麦条锈菌171bp和叶锈菌226 bp的特异性DNA片段,其检测灵敏度可达到50μg.L-1模板DNA浓度水平.在此基础上,可进一步制备小麦锈菌不同种的分子诊断、检测试剂盒.[著者文摘] In this research,a multiplex PCR analysis system was built successfully.The 171 b...