Multiplex PCR detection of wheat rust fungi

• Published in: Henan nongye daxue xuebao Vol. 44; no. 1
• Main Authors: Meng Haoguang, Henan Agricultural University,Zhengzhou(China), College of Plant Protection, Cao Lihua, Henan Agricultural University,Zhengzhou(China), College of Plant Protection, Zong Xianzhao, Henan Agricultural University,Zhengzhou(China), College of Plant Protection
• Format: Journal Article
• Language: Chinese
• Published: 01.01.2010
• Subjects: BLE; http://www.fao.org/aos/agrovoc#c_34079; http://www.fao.org/aos/agrovoc#c_6710; http://www.fao.org/aos/agrovoc#c_8373; PCR; ROUILLE; ROYA; RUSTS; TRIGO; WHEATS
• ISSN: 1000-2340

利用真菌β-微管蛋白基因的保守序列和小麦条锈菌（Puccinia striiformis f.sp.tritici）、叶锈菌（Puccinia tri-ticina）的SCAR标记引物,对锈菌孢子与感病小麦叶片总DNA进行复合PCR检测,扩增获得了小麦条锈菌171bp和叶锈菌226 bp的特异性DNA片段,其检测灵敏度可达到50μg.L-1模板DNA浓度水平.在此基础上,可进一步制备小麦锈菌不同种的分子诊断、检测试剂盒.[著者文摘] In this research,a multiplex PCR analysis system was built successfully.The 171 bp specific DNA fragment of Puccinia striiformis f.sp.tritici and the 226 bp specific DNA fragment of Puccinia triticina were amplified from the corresponding urediospores and the infected wheat leaves.The detection sensitivity of this multiplex PCR was 50 μg・L-1 template DNA.On this basis,a high-through kit can be further developed for the rapid diagnosis and detection of wheat rust fungi.[著者文摘]