SNP genotyping methods for crops in post-genomic era

• Published in: Molecular plant breeding Vol. 8; no. 1
• Main Authors: Zhao Qiongyi, Chinese Academy of Sciences, Beijing(China), Institute of Plant Physiology and Ecology, Li Xin, Shanghai JiaoTong University, Shanghai(China), School of Life Science and Biotechnology, Zhou Degui, The Rice Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou(China)
• Format: Journal Article
• Language: Chinese
• Published: 01.01.2010
• Subjects: CROPS; CULTIVOS; GENOMICA; GENOMICS; GENOMIQUE; GENOTIPOS; GENOTYPE; GENOTYPES; http://www.fao.org/aos/agrovoc#c_1972; http://www.fao.org/aos/agrovoc#c_3225; http://www.fao.org/aos/agrovoc#c_92382; PLANTE DE CULTURE
• ISSN: 1672-416X

单核苷酸多态性（single nueleotide polymorphisms，SNPs）是生物体最普遍的一种多态差异，在植物功能基因组研究和作物遗传改良方面有着广泛的应用。利用全基因组水平SNP标记谱进行遗传变异的研究、群体结构分析、关联性分析、作物分子设计育种，以及对大规模SNP数据进行验证、评估等，都迫切需要发展和利用各种不同的SNP分型手段实现。本文综述了目前常用的一些SNP分型方法，简要介绍了检测原理及操作流程，并对后基因组时代下作物的高通量SNP数据的分析进行了讨论。[著者文摘] Single Nucleotide Polymorphisms （SNPs） are the most widespread DNA sequence variation in organisms and widely applied in plant functional genomics and genetic improvement for crops. It is essential to use some feasible SNP genotyping approaches in assessing the quality of those data. For crops, it is important to identify and validate SNP markers that are needed for study in many fields, such as genetic variation, population structure analysis, association study, crop plants breeding, etc., This mini review focused on some commonly SNP genotyping methods, and gave a brief introduction to their detection principle and operation flow, then discussed the analysis of large amounts of SNP data for crops in post-genomic era.