A new sampling approach to obtain genomic DNA of marine shellfish

• Published in: Hunan agricultural science & technology newsletter : HASTN Vol. 11; no. 7
• Main Authors: Cai Xiaohui, Guangxi Institute of Oceanology, Beihai（China）, Song Zhongkui, Guangxi Institute of Oceanology, Beihai（China）, Peng Yinhui, Guangxi Institute of Oceanology, Beihai（China）
• Format: Journal Article
• Language: Chinese
• Published: 01.12.2010
• Subjects: ADN; CONCHA; COQUILLE; DNA; FILOGENIA; http://www.fao.org/aos/agrovoc#c_13325; http://www.fao.org/aos/agrovoc#c_2347; http://www.fao.org/aos/agrovoc#c_7032; PHYLOGENIE; PHYLOGENY; SHELL
• ISSN: 1009-4229

[Objective] The aim was to explore a new sampling approach to obtain genomic DNA of marine shellfish, as well as to provide reference for the molecular biology research on precious shellfish. [Method] Meretrix meretrix, Atrina pectinata, Perna viridis, Crassostrea hongkongensis and Scapharca kagoshimensis were used as experimental materials and the genomic DNA of adductor muscle was taken as reference to extract the genomic DNA of shell cavity fluids with the conventional phenol-chloroform method. And then biophotometer, agarose gel electrophoresis, amplification and sequencing of the target fragments were used to examine the quality of genomic DNA. At the same time, the phylogenic tree was constructed to verify the reality of source contributions. [Result] The quality of genomic DNA of shell cavity fluids extracted by the phenol-chloroform method was better than the genomic of adductor muscle. The genomic DNA extracted by this method showed less content of protein, polyphenol and pigment, which could complet