PCR-DGGE and its application in the research of environmental microbiology

变性梯度凝胶电泳（DGGE）技术目前已被广泛应用于环境微生物领域。介绍了DGGE的基本原理和技术步骤，分析了该方法的主要影响因素及其优点和存在的问题；概述了PCR-DGGE技术在环境微生物领域，包括在微生物处理技术、微生物生态多样性研究和功能菌种优化筛选中的应用现状，并展望了其应用前景。 The denaturing gradient gel electrophoresis (DGGE) has been proven to be widely used in the microbial environment, including the microbial treatment, micro...

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Bibliographic Details
Published inXi bei nong lin ke ji da xue xue bao.Zi ran ke xue ban Vol. 38; no. 6
Main Authors Yu jie, Tianjin University of Technology, Tianjin (China), College of Environmental Science and Safety Engineering, Feng xin, Tianjin University of Technology, Tianjin (China), College of Environmental Science and Safety Engineering, Xie Yuhong, Tianjin University of Technology, Tianjin (China), College of Environmental Science and Safety Engineering
Format Journal Article
LanguageChinese
Published 01.06.2010
Subjects
ENVIRONMENT
ENVIRONNEMENT
http://www.fao.org/aos/agrovoc#c_2593
http://www.fao.org/aos/agrovoc#c_34079
http://www.fao.org/aos/agrovoc#c_4800
MEDIO AMBIENTE
MICROBIOLOGIA
MICROBIOLOGIE
MICROBIOLOGY
PCR
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Summary:变性梯度凝胶电泳（DGGE）技术目前已被广泛应用于环境微生物领域。介绍了DGGE的基本原理和技术步骤，分析了该方法的主要影响因素及其优点和存在的问题；概述了PCR-DGGE技术在环境微生物领域，包括在微生物处理技术、微生物生态多样性研究和功能菌种优化筛选中的应用现状，并展望了其应用前景。 The denaturing gradient gel electrophoresis (DGGE) has been proven to be widely used in the microbial environment, including the microbial treatment, microbial diversity and the screening of the function bacterium. In this paper, the principle and technical route of DGGE is briefly introduced, including its main causing factors, the excellence and the limitation. The future of this technology is also expected.
Bibliography:F40
2011000217
ISSN:1671-9387