Study on Agrobacterium tumefaciens-mediated transformation of Brassica campestris L. with fusion gene Ycoil-bFGF

• Published in: Hunan agricultural science & technology newsletter : HASTN Vol. 10; no. 4
• Main Authors: Xu Yan, Ministry of Education, Engineering Research Center of Bioreactor and Pharmaceut Development, Changchun (China), Xiao Yanshuang, Jilin Agricultural University, Changchun(China), College of Chinese Medicinal Materials, Du Jinxia, Jilin Agricultural University, Changchun(China), College of Chinese Medicinal Materials
• Format: Journal Article
• Language: English
• Published: 01.08.2009
• Subjects: AGROBACTERIUM; BRASSICA CAMPESTRIS; GENE TRANSFER; http://www.fao.org/aos/agrovoc#c_1062; http://www.fao.org/aos/agrovoc#c_206; http://www.fao.org/aos/agrovoc#c_24846; TRANSFERENCIA DE GENES; TRANSFERT DE GENE
• ISSN: 1009-4229

[Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Method] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF was introduced into the rapeseed (Brassica campestris L.) by Agrobacterium tumefaciens-mediated transformation; meanwhile regeneration conditions of rapeseed were also optimized, and the regenerated resistant plantlets were detected by PCR and Southern blot. [Result] This fusion gene had been integrated into rapeseed genome successfully, and the optimized conditions of transformation and regeneration were as follows: explants pre-culture for 2 d, co-culture for 3 d, bacteria solution OD600 for 0.3 and infection time for 5 min. [Conclusion] The results laid a solid foundation for extraction, isolation and purification of protein in transgenic plant seeds.