Cloning and bioinformatics analysis of Lfcin gene of Datong Yak

[Objective] This study was to clone Lfcin gene from Datong yak, so as to provide reference for applying this gene in feed industry and breeding industry. [Method] Using PCR technology, the lactoferricin(Lfcin)-encoding gene was obtained from genome of Datong yak; then it was cloned into pGEM-T easy...

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Published inHunan agricultural science & technology newsletter : HASTN Vol. 10; no. 3
Main Authors Pei Jie, Chinese Academy of Agricultural Science, Lanzhou (China), Lanzhou Institute of Animal Science and Veterinary Pharmaceutical Science, Yan Ping, Chinese Academy of Agricultural Science, Lanzhou (China), Lanzhou Institute of Animal Science and Veterinary Pharmaceutical Science, Ji Guohong, Gansu Agricultural University, Lanzhou (China), College of Grass Science
Format Journal Article
LanguageEnglish
Published 01.06.2009
Subjects
BIOINFORMATICA
BIOINFORMATICS
BIOINFORMATIQUE
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
http://www.fao.org/aos/agrovoc#c_27503
http://www.fao.org/aos/agrovoc#c_37958
http://www.fao.org/aos/agrovoc#c_8477
MOLECULAR CLONING
YACK
YAK
YAKS
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Summary:[Objective] This study was to clone Lfcin gene from Datong yak, so as to provide reference for applying this gene in feed industry and breeding industry. [Method] Using PCR technology, the lactoferricin(Lfcin)-encoding gene was obtained from genome of Datong yak; then it was cloned into pGEM-T easy vector, and then sequenced; the sequencing results were subsequently aligned with the sequences of dairy cow accessed in GenBank. Moreover, amino acid sequences of Lfcin gene from various species including yak, dairy cow, human and mouse were used for sequence alignment and phylogenesis analysis. [Result] The second exon of lactoferrin(LF) from Datong yak, which is 778 bp in length, was obtained, within which the coding region of Lfcin gene is 75 bp (25 amino acid residues); sequence analysis showed that there is discrepancy of eleven bases between Datong yak and dairy cow; Lfcin proteins from various species shared high homeology, of which that from Datong yak and dairy cow were completely identical; phylogenesis analysis showed that cladogram based on Lfcin was consistent with species evolutionary law. [Conclusion] This study laid a foundation for the prokaryotic or eukaryotic expression of Lfcin gene and further understanding the activity of Lfcin protein.
Bibliography:L10
2010001211
ISSN:1009-4229