An effective method for extracting total RNA from young embryo of seedless litchi

A total RNA extraction method for young embryo of seedless litchi was introduced. CTAB, Phenol (saturated with water), chloroform, Guanidine isothiocyanate were used as main extraction reagents. Polyphenolic compounds were removed effectively by added PVP into the extraction buffer solution. RNA was...

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Bibliographic Details
Published inHunan agricultural science & technology newsletter : HASTN Vol. 9; no. 1
Main Authors Liu Xingdi, Zheng Xueqin
Format Journal Article
LanguageChinese
Published 01.02.2008
Subjects
EXTRACCION
EXTRACTION
GINECEO
GYNECEE
GYNOECIUM
http://www.fao.org/aos/agrovoc#c_33543
http://www.fao.org/aos/agrovoc#c_3451
http://www.fao.org/aos/agrovoc#c_36910
SEEDLESS VARIETIES
VARIEDADES ASPERMIAS
VARIETE APYRENE
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Summary:A total RNA extraction method for young embryo of seedless litchi was introduced. CTAB, Phenol (saturated with water), chloroform, Guanidine isothiocyanate were used as main extraction reagents. Polyphenolic compounds were removed effectively by added PVP into the extraction buffer solution. RNA was purified intensively by phenol, chloroform extraction, and ethanol deposition after deposited by LiCl. Both the results of formaldehyde denatured agarose gel electrophoresis and ultraviolet spectrophotometer analysis showed high integrity and purity of RNA. So the quality of extracted RNA could meet the demand of most molecular biology experiments that require higher quality RNA.
Bibliography:F30
2009001815
ISSN:1009-4229