Study on McAB sandwich ELISA for the detection of EDSV

Sandwich enzyme-linked immunosorbent assay (elisa) for detecting egg drop syndrome virus was developed by using three strains mono-clonal antibodies (McAbs). The optimal coating concentration of McAbs was 2 ug/ml working concentration of McAb-HRP was 1:400. The sensitivity of the McAb sandwich elisa...

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Bibliographic Details
Published inZhongguo shouyi zazhi Vol. 24; no. 3
Main Authors Wang Xuemin, Cai Baoxiang, Zheng Mingqiu(Handan Agricultural Coll., Yongnian(China) Coll. of Veterinary Medicine)
Format Journal Article
LanguageChinese
Published 01.03.1998
Subjects
CHICKENS
DESEMPENO EN LA POSTURA
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
ELISA
LAYING PERFORMANCE
PERFORMANCE DE PONTE
POLLO
POULET
TEST ELISA
VIROSE
VIROSES
VIROSIS
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Summary:Sandwich enzyme-linked immunosorbent assay (elisa) for detecting egg drop syndrome virus was developed by using three strains mono-clonal antibodies (McAbs). The optimal coating concentration of McAbs was 2 ug/ml working concentration of McAb-HRP was 1:400. The sensitivity of the McAb sandwich elisa was 0.03 ug/ml. The assay had no cross reaction with common pathogenic microorganisms. 50 cloaca swab samples from infected birds were detected, resulting in positive rate of 84 (42/50).
Bibliography:1999083602
L02
ISSN:0529-6005