Study on McAB sandwich ELISA for the detection of EDSV
Sandwich enzyme-linked immunosorbent assay (elisa) for detecting egg drop syndrome virus was developed by using three strains mono-clonal antibodies (McAbs). The optimal coating concentration of McAbs was 2 ug/ml working concentration of McAb-HRP was 1:400. The sensitivity of the McAb sandwich elisa...
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Published in | Zhongguo shouyi zazhi Vol. 24; no. 3 |
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Main Authors | , , |
Format | Journal Article |
Language | Chinese |
Published |
01.03.1998
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Subjects | |
Online Access | Get more information |
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Summary: | Sandwich enzyme-linked immunosorbent assay (elisa) for detecting egg drop syndrome virus was developed by using three strains mono-clonal antibodies (McAbs). The optimal coating concentration of McAbs was 2 ug/ml working concentration of McAb-HRP was 1:400. The sensitivity of the McAb sandwich elisa was 0.03 ug/ml. The assay had no cross reaction with common pathogenic microorganisms. 50 cloaca swab samples from infected birds were detected, resulting in positive rate of 84 (42/50). |
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Bibliography: | 1999083602 L02 |
ISSN: | 0529-6005 |