Cultivation of pasteurellas by different methods and their biological properties

• Published in: Ученые записки Учреждения образования Витебская государственная академия ветеринарной медицины : научно-практический журнал Vol. 46; no. 1; pp. 92 - 94
• Main Authors: Medvedev, A.P, Koshnerova, L.A, Gvozdev, S.N., Vitebsk State Academy of Veterinary Medicine (Belarus)
• Format: Journal Article
• Language: Russian
• Published: 2010
• Subjects: BACTA©RIOSE; BACTERIOSES; BACTERIOSIS; BA©LARUS; BELARUS; CULTIVATION; CULTIVO; CULTURE MEDIA; http://www.fao.org/aos/agrovoc#c_10204; http://www.fao.org/aos/agrovoc#c_2018; http://www.fao.org/aos/agrovoc#c_28464; http://www.fao.org/aos/agrovoc#c_330705; http://www.fao.org/aos/agrovoc#c_33237; http://www.fao.org/aos/agrovoc#c_36183; http://www.fao.org/aos/agrovoc#c_5622; http://www.fao.org/aos/agrovoc#c_770; MEDIO DE CULTIVO; MEDIO SELECTIVO; MILIEU DE CULTURE; MILIEU SA©LECTIF; PASTEURELLA; PASTEURELLOSE; PASTEURELLOSIS; PASTEURELOSIS; PRATIQUE CULTURALE; SELECTIVE MEDIA; SUBSTANCE; SUBSTANCES; SUSTANCIAS
• ISSN: 2078-0109

In the conditions of the Republic of Belarus there was realized a cultivation of pasteurellas by different methods in Hottinger broth in 37 deg C temperature in course of 24 hours. Tissue culture properties were studied in accordance with the character of bacteria growth in liquid nutritive media and at the agar surface. Research results showed that in 7 hours after cultivation in media with application of shuttel apparatus and addition of glucose it was possible to obtain the highest quantity of viable pasteurellas with a high degree of virulence. The obtained culture on the basis of obtained results was proposed to be used for the production of virulence antigen.