The formation of perhydroxy acid by immobilized lipoxygenase

Lipoxygenase-produced oxidized fatty acids may serve as intermediates in chemical and pharmaceutical synthesis. For a practical implementation of this production route the enzyme lipoxygenase should be immobilized to enable a continuous process and to prevent its loss along with product retrieval. I...

Full description

Saved in:
Bibliographic Details
Published inMededelingen - Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen. Universiteit Gent (Belgium) Vol. 59; no. 4b
Main Authors Bouwer, S.T, Kramer, G.F.H, Cuperus, F.P, Derksen, J.T.P. (Agrotechnological Research Inst., Wageningen (Netherlands))
Format Journal Article
LanguageEnglish
Published 1994
Subjects
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ANION
ANIONES
COMPOSE HYDROXYLE
COMPUESTOS DE HIDROXILO
ECHANGE D'ION
ENZIMAS INMOVILIZADAS
ENZYME IMMOBILISEE
HIDROFOBICIDAD
HYDROPHOBICITE
INDUSTRIA FARMACEUTICA
INDUSTRIE PHARMACEUTIQUE
INTERCAMBIO IONICO
LIPOXIGENASA
LIPOXYGENASE
Online AccessGet more information

Cover

More Information
Summary:Lipoxygenase-produced oxidized fatty acids may serve as intermediates in chemical and pharmaceutical synthesis. For a practical implementation of this production route the enzyme lipoxygenase should be immobilized to enable a continuous process and to prevent its loss along with product retrieval. In this study the immobilization of soybean lipoxygenase on various neutral and charged supports was investigated. The best results were obtained using cellulose based anion exchangers. The activity of the immobilized lipoxygenase was lower than the activity of the free enzyme, which may be caused by diffusion limitation of the fatty acids towards or from the support beads.
Bibliography:Q02
9500815
ISSN:0368-9697